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A novel prognosis evaluation indicator of patients with sepsis created by integrating six microfluidic-based neutrophil chemotactic migration parameters

文献类型：期刊论文

作者Yang, Xiao1,2; Pu, Xuexue3; Xu, Yuanyuan4; Zhao, Jun2; Fang, Xiao3; Cui, Junsheng2; Deng, Guoqing2; Liu, Yong2; Zhu, Ling2; Shao, Min3
刊名TALANTA
出版日期2025
卷号281
关键词Neutrophil Migration Microfluidic chip Sepsis patients Diagnosis and prognosis assessment
ISSN号0039-9140
DOI10.1016/j.talanta.2024.126801
通讯作者Shao, Min(shaomin@ahmu.edu.cn) ; Yang, Ke(keyang@aiofm.ac.cn)
英文摘要Impaired neutrophil migration in sepsis is associated with a poor prognosis. The potential of utilizing neutrophil chemotaxis to assess immune function, disease severity, and patient prognosis in sepsis remains underexplored. This study employed an innovative approach by integrating a multi-tip pipette with a Six-Unit microfluidic chip (SU6-chip) to establish gradients in six microchannels, thereby analyzing neutrophil chemotaxis in sepsis patients. We compared chemotactic parameters between healthy controls (NH = 20) and sepsis patients (NS1 = 25), observing significant differences in gradient perception time (GP), migration distance (MD), peak velocity (Vmax), chemotactic index (CI), reverse migration rate (RM), and stop migration number (SM). A novel composite indicator, the Sepsis Neutrophil Migration Evaluation (SNME) index, was developed by integrating these six chemotactic migration parameters. The SNME index and individual chemotaxis parameters showed significant correlations with the Sequential Organ Failure Assessment (SOFA) score, Acute Physiology and Chronic Health Evaluation (APACHE II) score, hypersensitivity C-reactive protein (hs-CRP), and heparin-binding protein (HBP). Moreover, the SNME index demonstrated potential for monitoring sepsis progression, with ROC analysis confirming its predictive accuracy (area under the curve [AUC] = 0.895, cutoff value = 31.5, specificity = 86.73 %, sensitivity = 86.71 %), outperforming individual neutrophil chemotactic parameters. In conclusion, the SNME index represents a promising new tool for adjunctive diagnosis and prognosis assessment in patients with sepsis.
WOS关键词EARLY-DIAGNOSIS ; SEPTIC SHOCK ; NITRIC-OXIDE ; EXPRESSION ; COAGULATION ; MONOCYTES ; RECEPTOR ; PROTEIN ; ASSAY
资助项目National Natural Science Foun-dation of China[31800713] ; Youth Innovation Promotion As-sociation of the Chinese Academy of Sciences[2021442] ; Anhui Provincial Natural Science Foundation[2308085MC75] ; Major Project from Innovation Research Institute of Engineering Medi-cine and Medical Equipment, Hefei Comprehensive National Science Center[HFGJKXZX-YGRH-1-005]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:001308741900001
出版者ELSEVIER
资助机构National Natural Science Foun-dation of China ; Youth Innovation Promotion As-sociation of the Chinese Academy of Sciences ; Anhui Provincial Natural Science Foundation ; Major Project from Innovation Research Institute of Engineering Medi-cine and Medical Equipment, Hefei Comprehensive National Science Center
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/135167]  
专题中国科学院合肥物质科学研究院
通讯作者Shao, Min; Yang, Ke
作者单位1.Univ Sci & Technol China, Hefei 230026, Peoples R China
2.Chinese Acad Sci, Anhui Inst Opt & Fine Mech, Hefei Inst Phys Sci, Hefei 230031, Anhui, Peoples R China
3.Anhui Med Univ, Dept Pediat, Affiliated Hosp 1, Hefei 230022, Peoples R China
4.Anhui Med Univ, Dept Pediat Crit Care Med, Childrens Med Ctr, Hefei 230051, Peoples R China
推荐引用方式
GB/T 7714		 Yang, Xiao,Pu, Xuexue,Xu, Yuanyuan,et al. A novel prognosis evaluation indicator of patients with sepsis created by integrating six microfluidic-based neutrophil chemotactic migration parameters[J]. TALANTA,2025,281.
APA Yang, Xiao.,Pu, Xuexue.,Xu, Yuanyuan.,Zhao, Jun.,Fang, Xiao.,...&Yang, Ke.(2025).A novel prognosis evaluation indicator of patients with sepsis created by integrating six microfluidic-based neutrophil chemotactic migration parameters.TALANTA,281.
MLA Yang, Xiao,et al."A novel prognosis evaluation indicator of patients with sepsis created by integrating six microfluidic-based neutrophil chemotactic migration parameters".TALANTA 281(2025).

入库方式： OAI收割

来源：合肥物质科学研究院

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