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Mutation of KAP, which encodes a keratin-associated protein, affects grain size and yield production in rice

文献类型：期刊论文

作者Chen, Chunpeng2,3; Cheng, Weimin2; Jiang, Hongrui2,3; Fang, Cheng2,3; Li, Wenhao2; Peng, Lingling2; Tao, Liangzhi2; Zhan, Yue2; Wu, Yuejin2; Huang, Xianzhong1
刊名PHYSIOLOGIA PLANTARUM
出版日期2024-09-01
卷号176
ISSN号0031-9317
DOI10.1111/ppl.14528
通讯作者Huang, Xianzhong(huangxz@ahstu.edu.cn) ; Liu, Binmei(liubm@ipp.ac.cn) ; Ye, Yafeng(yyfeng86@163.com)
英文摘要Grain size and shape are critical agronomic traits that directly impact rice grain yield. Identifying genes that control these traits can provide new strategies for yield improvement. In this study, we characterized a rice mutant, reduced grain length (rgl), which exhibited decreased grain length due to reduced cell proliferation. Map-based cloning identified a base deletion in OsRGL2, a gene encoding a keratin-associated protein (KAP), as the cause of the mutant phenotype. CRISPR-Cas9-generated OsRGL2 knockout mutants also displayed reduced grain length, confirming its role. OsRGL2 transcripts were detected in various tissues, with relative higher gene expression in young panicles, and OsRGL2 was localized to the plasma membrane. Overexpression of OsRGL2 increased grain size by promoting cell proliferation in the spikelet hull and significantly enhanced grain yield per plant. Importantly, OsRGL2 was found to interact with RGB1, indicating that OsRGL2 positively regulates grain size and yield through its interaction with RGB1. Additionally, OsRGL2 regulated the expression of cell cycle-related genes, further elucidating its role in grain development. These findings demonstrate that OsRGL2 is a positive regulator of grain size in rice, and manipulating its expression may offer a novel strategy for enhancing rice grain yield.
WOS关键词NATURAL VARIATION ; WALL BIOSYNTHESIS ; MAJOR QTL ; WIDTH ; SHAPE ; EFFICIENCY ; SUBUNIT ; WEIGHT ; NUMBER ; SEED
资助项目Youth Innovation Promotion Association CAS (2022454)[2023n06020006] ; Key Research and Developmental Program of Anhui Province[2022454] ; Youth Innovation Promotion Association CAS[32171962] ; Youth Innovation Promotion Association CAS[U23A20183] ; National Natural Science Foundation of China[2023YFD1901005] ; National Key Research and Developmental Program of China[YZJJKX202201] ; HFIPS Director's Fund[2022AH010087] ; HFIPS Director's Fund[B23CQ15IP] ; Excellent scientific research and innovation team of the Education Department of Anhui Province
WOS研究方向Plant Sciences
语种英语
WOS记录号WOS:001313877800001
出版者WILEY
资助机构Youth Innovation Promotion Association CAS (2022454) ; Key Research and Developmental Program of Anhui Province ; Youth Innovation Promotion Association CAS ; National Natural Science Foundation of China ; National Key Research and Developmental Program of China ; HFIPS Director's Fund ; Excellent scientific research and innovation team of the Education Department of Anhui Province
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/135212]  
专题中国科学院合肥物质科学研究院
通讯作者Huang, Xianzhong; Liu, Binmei; Ye, Yafeng
作者单位1.Anhui Sci & Technol Univ, Coll Agr, Ctr Crop Biotechnol, Chuzhou, Peoples R China
2.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei, Peoples R China
3.Univ Sci & Technol China, Hefei, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Chunpeng,Cheng, Weimin,Jiang, Hongrui,et al. Mutation of KAP, which encodes a keratin-associated protein, affects grain size and yield production in rice[J]. PHYSIOLOGIA PLANTARUM,2024,176.
APA Chen, Chunpeng.,Cheng, Weimin.,Jiang, Hongrui.,Fang, Cheng.,Li, Wenhao.,...&Ye, Yafeng.(2024).Mutation of KAP, which encodes a keratin-associated protein, affects grain size and yield production in rice.PHYSIOLOGIA PLANTARUM,176.
MLA Chen, Chunpeng,et al."Mutation of KAP, which encodes a keratin-associated protein, affects grain size and yield production in rice".PHYSIOLOGIA PLANTARUM 176(2024).

入库方式： OAI收割

来源：合肥物质科学研究院

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