中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 合肥物质科学研究院
  3. 中国科学院合肥物质科学研究院
Viral capsid-mediated efficient cell labeling of micron-sized magnetic particles for highly sensitive long-term in vivo tumor cells MRI tracking

文献类型：期刊论文

作者Feng, Xiayi2,7; Gao, Ding2; Zhou, Juan2; Gui, Zhu1; Cai, Aoling8; Wang, Jie9; Dai, Ji10; Jing, Yipeng11; Qian, Junchao12; Cui, Zongqiang2
刊名CHEMICAL ENGINEERING JOURNAL
出版日期2024-10-15
卷号498
关键词Magnetic resonance imaging Micron-sized iron oxide particles Virus-like nanoparticles Single cell Long-term imaging
ISSN号1385-8947
DOI10.1016/j.cej.2024.155830
通讯作者Zhang, Xian-En(zhangxe@ibp.ac.cn) ; Men, Dong(men_dong@gzlab.ac.cn)
英文摘要As a contrast agent, micron-sized iron oxide particles (MPIO) offer major advantages for monitoring tumor cells in vivo -applications crucial for studies of tumorigenesis and metastasis. However, labeling tumor cells with MPIO is often dependent on phagocytosis, which limits the labeling efficiency of the cells and thus affects in vivo tumor cell tracking. To address this issue, we prepared a virus-like particle (VLP) consisting of the SV40 VP1 protein modified on the surface of a MPIO (vMPIO). The vMPIO can enter cells via a pathway similar to that of viral infection (caveolae-dependent endocytosis pathway). This approach increased the number of vMPIO in individual labeled cells, which in turn enhances magnetic resonance imaging (MRI) signal intensity and the number of vMPIO in daughter cells. These vMPIO-labeled cells can be detected by MRI in vitro and in vivo even in very small numbers (<= 10 <= 10 cells). Furthermore, vMPIO-labeled cells were successfully applied to long-term tracing of tumor tissue and detection of early metastatic lesions. This VLP-mediated MPIO labeling strategy (vML) not only enables highly sensitive in vivo MRI but also introduces a useful technical tool for investigating tumor invasion and early metastasis.
WOS关键词DELIVERY ; GM1 ; GANGLIOSIDE ; CARCINOMA ; STEM
资助项目National Key Research and Development Program of China[2019YFA0904801] ; Major Project of Guangzhou National Laboratory[SRPG22-020] ; National Natural Science Foundaton of Chin[31771098] ; Youth Innovation Promotion Association of CAS[2014308] ; Youth Innovation Promotion Association of CAS[2023352]
WOS研究方向Engineering
语种英语
WOS记录号WOS:001318438600001
出版者ELSEVIER SCIENCE SA
资助机构National Key Research and Development Program of China ; Major Project of Guangzhou National Laboratory ; National Natural Science Foundaton of Chin ; Youth Innovation Promotion Association of CAS
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/135485]  
专题中国科学院合肥物质科学研究院
通讯作者Zhang, Xian-En; Men, Dong
作者单位1.Chinese Acad Sci, Wuhan Natl Lab Optoelect,Innovat Acad Precis Measu, Wuhan Inst Phys & Math,Natl Ctr Magnet Resonance W, State Key Lab Magnet Resonance & Atom & Mol Phys,K, Wuhan 430071, Peoples R China
2.Chinese Acad Sci, Wuhan Inst Virol, Ctr Biosafety Mega Sci, State Key Lab Virol, Wuhan 430071, Peoples R China
3.Guangzhou Natl Lab, Guangzhou 510005, Peoples R China
4.Guangzhou Med Univ, Affiliated Hosp 1, Guangzhou Inst Resp Dis, State Key Lab Resp Dis, Guangzhou 510182, Guangdong, Peoples R China
5.Shenzhen Univ Adv Technol, Fac Synthet Biol, Shenzhen 518055, Peoples R China
6.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China
7.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
8.Nanjing Med Univ, Changzhou Peoples Hosp 2, Changzhou Med Ctr, Changzhou 213100, Jiangsu, Peoples R China
9.Shanghai Jiao Tong Univ, Songjiang Hosp, Sch Med, Songjiang Res Inst, Shanghai 200025, Peoples R China
10.Chinese Acad Sci, Shenzhen Hong Kong Inst Brain Sci, Shenzhen Inst Adv Technol, Shenzhen Technol Res Ctr Primate Translat Med, Shenzhen 518055, Peoples R China
推荐引用方式
GB/T 7714		 Feng, Xiayi,Gao, Ding,Zhou, Juan,et al. Viral capsid-mediated efficient cell labeling of micron-sized magnetic particles for highly sensitive long-term in vivo tumor cells MRI tracking[J]. CHEMICAL ENGINEERING JOURNAL,2024,498.
APA Feng, Xiayi.,Gao, Ding.,Zhou, Juan.,Gui, Zhu.,Cai, Aoling.,...&Men, Dong.(2024).Viral capsid-mediated efficient cell labeling of micron-sized magnetic particles for highly sensitive long-term in vivo tumor cells MRI tracking.CHEMICAL ENGINEERING JOURNAL,498.
MLA Feng, Xiayi,et al."Viral capsid-mediated efficient cell labeling of micron-sized magnetic particles for highly sensitive long-term in vivo tumor cells MRI tracking".CHEMICAL ENGINEERING JOURNAL 498(2024).

入库方式： OAI收割

来源：合肥物质科学研究院

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。