Rational Design of an Artificial Metalloenzyme by Constructing a Metal-Binding Site Close to the Heme Cofactor in Myoglobin
文献类型：期刊论文
| 作者 | Nie, Lv-Suo1; Liu, Xi-Chun1; Yu, Lu2; Liu, Ao-Kun2; Sun, Li-Juan3; Gao, Shu-Qin3; Lin, Ying-Wu1,3 |
| 刊名 | INORGANIC CHEMISTRY
 |
| 出版日期 | 2024-09-23 |
| 卷号 | 63 |
| ISSN号 | 0020-1669 |
| DOI | 10.1021/acs.inorgchem.4c03093 |
| 通讯作者 | Lin, Ying-Wu(ywlin@usc.edu.cn) |
| 英文摘要 | In this study, we constructed a metal-binding site close to the heme cofactor in myoglobin (Mb) by covalently attaching a nonnative metal-binding ligand of bipyridine to Cys46 through the F46C mutation in the heme distal site. The X-ray structure of the designed enzyme, termed F46C-mBpy Mb, was solved in the Cu(II)-bound form, which revealed the formation of a heterodinuclear center of Cu-His-H2O-heme. Cu(II)-F46C-mBpy Mb exhibits not only nitrite reductase reactivity but also cascade reaction activity involving both hydrolysis and oxidation. Furthermore, F46C-mBpy Mb displays Mn-peroxidase activity by the oxidation of Mn2+ to Mn3+ using H2O2 as an oxidant. This study shows that the construction of a nonnative metal-binding site close to the heme cofactor is a convenient approach to creating an artificial metalloenzyme with a heterodinuclear center that confers multiple functions. |
| WOS关键词 | NITRIC-OXIDE ; MANGANESE PEROXIDASE ; COPPER ; ACTIVATION ; REDUCTION ; CHEMISTRY ; OXIDASES |
| 资助项目 | National Natural Science Foundation of China[32171270] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001320853300001 |
| 出版者 | AMER CHEMICAL SOC |
| 资助机构 | National Natural Science Foundation of China |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/135601]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Lin, Ying-Wu |
| 作者单位 | 1.Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China 2.Chinese Acad Sci, High Magnet Field Lab, Hefei 230031, Anhui, Peoples R China 3.Univ South China, Hengyang Med Sch, Hengyang 421001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Nie, Lv-Suo,Liu, Xi-Chun,Yu, Lu,et al. Rational Design of an Artificial Metalloenzyme by Constructing a Metal-Binding Site Close to the Heme Cofactor in Myoglobin[J]. INORGANIC CHEMISTRY,2024,63. |
| APA | Nie, Lv-Suo.,Liu, Xi-Chun.,Yu, Lu.,Liu, Ao-Kun.,Sun, Li-Juan.,...&Lin, Ying-Wu.(2024).Rational Design of an Artificial Metalloenzyme by Constructing a Metal-Binding Site Close to the Heme Cofactor in Myoglobin.INORGANIC CHEMISTRY,63. |
| MLA | Nie, Lv-Suo,et al."Rational Design of an Artificial Metalloenzyme by Constructing a Metal-Binding Site Close to the Heme Cofactor in Myoglobin".INORGANIC CHEMISTRY 63(2024). |
入库方式： OAI收割
来源：合肥物质科学研究院
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