Elucidating the protective mechanism of ganoderic acid DM on breast cancer based on network pharmacology and in vitro experimental validation
文献类型：期刊论文
| 作者 | Swallah, Mohammed Sharif1,2; Bondzie-Quaye, Precious1,2; Yu, Xin1,2; Fetisoa, Monia Ravelonandrasana1,2; Shao, Chang-Sheng3; Huang, Qing1,2
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| 刊名 | BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
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| 出版日期 | 2024-09-25 |
| 关键词 | alternative drugs breast cancer ganoderic acids Ganoderma lucidum molecular docking network pharmacology |
| ISSN号 | 0885-4513 |
| DOI | 10.1002/bab.2673 |
| 通讯作者 | Shao, Chang-Sheng(shaochangsheng@hmfl.ac.cn) ; Huang, Qing(huangq@ipp.ac.cn) |
| 英文摘要 | Ganoderma lucidum, a popular medicinal fungus, has been utilized to treat a variety of diseases. It possesses a unique therapeutic and pharmacological reputation in suppressing cancer/tumor progression, especially breast cancer, due to its embedded rich bioactive chemical constituents, mainly triterpenoids (ganoderic acids). The most prevalent malignant tumor in women with a high mortality and morbidity rate is breast cancer. Ganoderic acids A, D, DM, F, and H are evidenced in previous research to have breast cancer-preventive properties by exhibiting autophagic and apoptosis, anti-proliferative, and anti-angiogenesis effects. However, the anti-breast cancer mechanism remains unclear. The putative targets of the ganoderic acids were further determined using bioinformatics techniques and molecular docking calculation. Finally, the key targets were verified in vitro. A total of 53 potential target proteins associated with 202 pathways were predicted to be related to breast cancer. The potential targets were narrowed down to six key targets (AKT1, PIK3CA, epidermal growth factor receptor [EGFR], STAT1, ESR1, and CTNNB1), using different algorithms of the CytoHubba plugin, which were further validated using molecular docking analysis. The ganoderic acid DM (GADM) and the targets (PIK3CA and EGFR) with the strongest interactions were validated via MDA-MB-231 and MCF7 cells. The expression level of PIK3CA in both MDA-MB-231 and MCF7 cells was dose-dependently suppressed by GADM, whereas EGFR expression was unexpectedly increased, which warrants further investigation. These data indicated that the network pharmacology-based prediction of GADM targets for treating human breast cancer could be reliable. |
| WOS关键词 | CELL-CYCLE ARREST ; INVASIVE BREAST ; LUCIDUM ; EXTRACT ; APOPTOSIS ; UPDATE |
| 资助项目 | National Natural Science Foundation of China[11635013] ; Hefei Mycological Valley Innovation Research Institute[jwg202305] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:001319512500001 |
| 出版者 | WILEY |
| 资助机构 | National Natural Science Foundation of China ; Hefei Mycological Valley Innovation Research Institute |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/135619]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Shao, Chang-Sheng; Huang, Qing |
| 作者单位 | 1.Chinese Acad Sci, Inst Intelligent Machines, Hefei Inst Phys Sci, CAS Key Lab High Magnet Field & Iron Beam Phys Bio, Hefei 230031, Peoples R China 2.Univ Sci & Technol China, Sci Isl Branch, Grad Sch, Hefei, Peoples R China 3.Chinese Acad Sci, Hefei Inst Phys Sci, High Magnet Field Lab, Hefei 230031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Swallah, Mohammed Sharif,Bondzie-Quaye, Precious,Yu, Xin,et al. Elucidating the protective mechanism of ganoderic acid DM on breast cancer based on network pharmacology and in vitro experimental validation[J]. BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,2024. |
| APA | Swallah, Mohammed Sharif,Bondzie-Quaye, Precious,Yu, Xin,Fetisoa, Monia Ravelonandrasana,Shao, Chang-Sheng,&Huang, Qing.(2024).Elucidating the protective mechanism of ganoderic acid DM on breast cancer based on network pharmacology and in vitro experimental validation.BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY. |
| MLA | Swallah, Mohammed Sharif,et al."Elucidating the protective mechanism of ganoderic acid DM on breast cancer based on network pharmacology and in vitro experimental validation".BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY (2024). |
入库方式： OAI收割
来源：合肥物质科学研究院
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