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Mobile lab: A novel pathogen assay using the nucleic acid automatic assay system assisted by a self-contained microfluidic cassette and chitosan decorating magnetic particles

文献类型：期刊论文

作者Yang, Ke4; Zhou, Jiujian1,4; Zhao, Jun4; Liu, Lijuan2,4; Hua, Changyi4; Hong, Chenggang3,4; Wang, Minglong3,4; Hu, Anzhong3,4; Zhang, Weilong3,4; Cui, Junsheng4
刊名SENSORS AND ACTUATORS B-CHEMICAL
出版日期2024-11-15
卷号419
关键词Microfluidic cassette Chitosan decorating magnetic particles Nucleic acid automatic assay system Pathogens assay "in-situPCR"
DOI10.1016/j.snb.2024.136413
通讯作者Yang, Ke(keyang@aiofm.ac.cn) ; Zhu, Ling(zhul@aiofm.ac.cn)
英文摘要Microfluidic pathogen detection cartridges with integrated switching valves have gained significant attention. Traditionally, these microfluidic cartridges utilized magnetic beads for a "bind-wash-elute-reaction" protocol to extract DNA but often lacked PCR-based aerosol contamination validation and focused primarily on isothermal applications. There is room to improve these systems, such as using novel DNA extraction methods and integrating PCR as the gold standard. In this study, we developed chitosan-decorated magnetic (CDM) particles and incorporated them into a mass-produced, self-contained microfluidic cartridge for the first time. Our results demonstrated that the DNA can be efficiently enriched from the lysis buffer (pH 5) using 75 % degree of deacetylation and 1 % of chitosan-modified CDM particles (20 mu L). The DNA extraction efficiency exceeded 90 %. The CDM particles enabled "in-situ PCR", simplifying the on-chip detection process to a "bind-wash-reaction" protocol. Additionally, we developed a nucleic acid automatic assay system (NA(3) system) featuring a fluid thermocycler, four-channel optical detection, magnet, microcontroller, and software. The entire assay, from sample preparation to detection, can be completed within approximately 60 minutes, simultaneously detecting 16 targets. The performance of the NA(3) system was better than that of the pristine magnetic microparticles-based assay and the commercial magnetic beads kit-based assay. The linearity, specificity, multi-pathogen detection, and user-friendliness of the NA(3) system were tested. We validated the NA(3) system's performance in detecting food-borne and serum infectious pathogens, demonstrating its potential for use in resource-limited settings.
WOS关键词POLYMERASE-CHAIN-REACTION ; BLOOD
资助项目National Key Research and Devel-opment Program of China[2022YFC2302700] ; National Key Research and Devel-opment Program of China[2022YFC2302703] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences[2021442] ; Anhui Provincial Key Research and Development Project[2023s07020004]
WOS研究方向Chemistry ; Electrochemistry ; Instruments & Instrumentation
语种英语
WOS记录号WOS:001319984300001
出版者ELSEVIER SCIENCE SA
资助机构National Key Research and Devel-opment Program of China ; Youth Innovation Promotion Association of the Chinese Academy of Sciences ; Anhui Provincial Key Research and Development Project
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/135640]  
专题中国科学院合肥物质科学研究院
通讯作者Yang, Ke; Zhu, Ling
作者单位1.Anhui Med Univ, Sch Biomed Engn, Hefei 230032, Anhui, Peoples R China
2.Univ Sci & Technol China, Hefei 230026, Anhui, Peoples R China
3.Hefei Zhongke Yikangda Biomed Co LTD, Hefei 230032, Anhui, Peoples R China
4.Chinese Acad Sci, Anhui Inst Opt & Fine Mech, Hefei Inst Phys Sci, Hefei 230032, Anhui, Peoples R China
推荐引用方式
GB/T 7714		 Yang, Ke,Zhou, Jiujian,Zhao, Jun,et al. Mobile lab: A novel pathogen assay using the nucleic acid automatic assay system assisted by a self-contained microfluidic cassette and chitosan decorating magnetic particles[J]. SENSORS AND ACTUATORS B-CHEMICAL,2024,419.
APA Yang, Ke.,Zhou, Jiujian.,Zhao, Jun.,Liu, Lijuan.,Hua, Changyi.,...&Zhu, Ling.(2024).Mobile lab: A novel pathogen assay using the nucleic acid automatic assay system assisted by a self-contained microfluidic cassette and chitosan decorating magnetic particles.SENSORS AND ACTUATORS B-CHEMICAL,419.
MLA Yang, Ke,et al."Mobile lab: A novel pathogen assay using the nucleic acid automatic assay system assisted by a self-contained microfluidic cassette and chitosan decorating magnetic particles".SENSORS AND ACTUATORS B-CHEMICAL 419(2024).

入库方式： OAI收割

来源：合肥物质科学研究院

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