Resveratrol enhances the antiliver cancer effect of cisplatin by targeting the cell membrane protein PLA2
文献类型：期刊论文
| 作者 | Gao, Yu2; Yang, Zhanyi2; Bajpai, Akhilesh Kumar1; Wang, Wenben2; Zhang, Liyuan2; Xia, Zhenhong2,3 |
| 刊名 | FRONTIERS IN ONCOLOGY
 |
| 出版日期 | 2024-09-24 |
| 卷号 | 14 |
| 关键词 | combination therapy resveratrol cisplatin anti-liver cancer mechanism cell membrane PLA2 |
| ISSN号 | 2234-943X |
| DOI | 10.3389/fonc.2024.1453164 |
| 通讯作者 | Xia, Zhenhong(xiazhenhong@bzmc.edu.cn) |
| 英文摘要 | Background In this study, we aimed to explore the mechanism by which resveratrol promotes cisplatin-induced death of HepG2 cells and to provide a potential strategy for resveratrol in the treatment of cancer.Methods HepG2 cells were exposed to a range of drug concentrations for 24 h: resveratrol (2.5 mu g/mL [10.95 mu M], 5 mu g/mL [21.91 mu M], 10 mu g/mL [43.81 mu M], 20 mu g/mL [87.62 mu M], 40 mu g/mL [175.25 mu M], and 80 mu g/mL [350.50 mu M]), cisplatin (0.625 mu g/mL [2.08 mu M], 1.25 mu g/mL [4.17 mu M], 2.5 mu g/mL [8.33 mu M], 4.5 mu g/mL [15.00 mu M], and 10 mu g/mL [33.33 mu M]), 24 mu g/mL (105.15 mu M) resveratrol + 9 mu g/mL (30.00 mu M) cisplatin, and 12 mu g/mL (52.57 mu M) resveratrol + 4.5 mu g/mL (15.00 mu M) cisplatin. The interaction of two drugs was evaluated by coefficient of drug interaction (CDI), which was based on the Pharmacological Additivity model. The MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to detect the effect of different concentrations of drugs on cell viability, while transcriptome sequencing was used to identify pathways associated with higher gene enrichment. Synchrotron radiation FTIR microspectroscopy experiments and data analysis were conducted to obtain detailed spectral information. The second-derivative spectra were calculated using the Savitzky-Golay algorithm. Single-cell infrared spectral absorption matrices were constructed to analyze the spectral characteristics of individual cells. The Euclidean distance between cells was calculated to assess their spectral similarity. The cell-to-cell Euclidean distance was computed to evaluate the spatial relationships between cells. The target protein of resveratrol was verified by performing a Western blot analysis.Results After 24 h of treatment with resveratrol, HepG2 cell growth was inhibited in a dose-dependent manner. Resveratrol promotes cisplatin-induced HepG2 cell death through membrane-related pathways. It also significantly changes the membrane components of HepG2 cells. Additionally, resveratrol changes the morphology of the HepG2 cell membrane by decreasing the expression of PLA2G2.Conclusion Resveratrol changes the morphology of the HepG2 cell membrane by decreasing the expression of PLA2G2 and promotes cisplatin-induced HepG2 cell death. The combination of cisplatin and resveratrol can play a synergistic therapeutic effect on HepG2 cells. |
| WOS关键词 | PROSTATIC INTRAEPITHELIAL NEOPLASIA ; IIA SECRETORY PHOSPHOLIPASE-A2 ; MESENCHYMAL STEM-CELLS ; CYTOREDUCTIVE SURGERY ; OVARIAN-CARCINOMA ; DRUG-RESISTANCE ; A(2) ENZYMES ; APOPTOSIS ; EXPRESSION ; DIFFERENTIATION |
| 资助项目 | Youth Foundation of (Natural Science Foundation of Shandong Province)[ZR2021QH305] ; Startup Foundation for Introducing Talent of Binzhou Medical University[BY2020KYQD24] |
| WOS研究方向 | Oncology |
| 语种 | 英语 |
| WOS记录号 | WOS:001327409600001 |
| 出版者 | FRONTIERS MEDIA SA |
| 资助机构 | Youth Foundation of (Natural Science Foundation of Shandong Province) ; Startup Foundation for Introducing Talent of Binzhou Medical University |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/135788]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Xia, Zhenhong |
| 作者单位 | 1.Univ Tennessee, Dept Genet Genom & Informat, Hlth Sci Ctr, Memphis, TN USA 2.Binzhou Med Univ, Dept Pharm, Yantai, Peoples R China 3.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab Ion Beam Bioengn, Hefei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Yu,Yang, Zhanyi,Bajpai, Akhilesh Kumar,et al. Resveratrol enhances the antiliver cancer effect of cisplatin by targeting the cell membrane protein PLA2[J]. FRONTIERS IN ONCOLOGY,2024,14. |
| APA | Gao, Yu,Yang, Zhanyi,Bajpai, Akhilesh Kumar,Wang, Wenben,Zhang, Liyuan,&Xia, Zhenhong.(2024).Resveratrol enhances the antiliver cancer effect of cisplatin by targeting the cell membrane protein PLA2.FRONTIERS IN ONCOLOGY,14. |
| MLA | Gao, Yu,et al."Resveratrol enhances the antiliver cancer effect of cisplatin by targeting the cell membrane protein PLA2".FRONTIERS IN ONCOLOGY 14(2024). |
入库方式： OAI收割
来源：合肥物质科学研究院
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