Efficient plant regeneration through direct shoot organogenesis and two-step rooting in Eucommia ulmoides Oliver
文献类型：期刊论文
| 作者 | Wang, Dacheng1,2; Su, Pengfei1,2; Gao, Yameng2; Chen, Xue2; Kan, Wenjie1,2; Hou, Jinyan2 ; Wu, Lifang1,2,3
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| 刊名 | FRONTIERS IN PLANT SCIENCE
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| 出版日期 | 2024-09-20 |
| 卷号 | 15 |
| 关键词 | adventitious root axillary bud sprouting E. ulmoides shoot elongation two-step rooting |
| ISSN号 | 1664-462X |
| DOI | 10.3389/fpls.2024.1444878 |
| 通讯作者 | Hou, Jinyan(jyhou@ipp.ac.cn) ; Wu, Lifang(lfwu@ipp.ac.cn) |
| 英文摘要 | Eucommia ulmoides Oliver (E. ulmoides Oliver), a multipurpose woody plant, holds great economic significance due to its expansive medicinal, food and industrial applications. The rapid advancement of E. ulmoides in various fields has resulted in the inadequacy of existing breeding methods to meet its growth and annual production demands. Consequently, there is an urgent need for innovative propagation strategies. This study introduces an optimized micropropagation protocol for E. ulmoides, facilitating direct shoot organogenesis from nodal segments with axillary buds. We systematically examined the impact of basal medium composition, plant growth regulators, photosynthetic photon flux density, and sucrose concentration on bud sprouting. Employing cuttings with axillary buds as propagation material, we achieved a shortened cultivation period of merely 4 weeks for bud elongation and proliferation, marking a substantial enhancement in propagation efficiency. Notably, the Driver Kuniyuki Walnut medium, supplemented with 20.0 g L-1 sucrose and 2.0 mg L-1 trans-zeatin, induced shoots sprouting with a 100% success rate and an average length of 5.18 cm per nodal segment, equating to a great bud propagation rate of approximately 500%. Furthermore, a light source with an intensity of 80 mu mol m(-2) s(-1) was shown the most economical choice. To address the primary challenge of inducing roots in regenerated plants, we employed a refined two-step rooting technique. This method yielded the optimal rooting frequency of 93.02%, producing an average of 5.90 adventitious roots per plantlet, each with an average length of 2.77 cm. The micropropagation program developed in this work will be the cornerstone for the preservation of the germplasm of E. ulmoides and its long-term use in medicinal and industrial applications. |
| WOS关键词 | RUBBER BIOSYNTHESIS ; SUSPENSION-CULTURES ; GROWTH ; SUCROSE ; TISSUE ; EUFPS1 ; GENE |
| 资助项目 | Strategic Biological Resources Planning Project of the Chinese Academy of Sciences[KFJ-BRP-007-014] ; Henan Provincial Major Science and Technology Project[21110110100] ; National Natural Science Foundation of China[32201521] ; Youth Innovation Promotion Association CAS[2021447] ; Key Research and Development Program of Anhui Province[202204c06020045] |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:001328712100001 |
| 出版者 | FRONTIERS MEDIA SA |
| 资助机构 | Strategic Biological Resources Planning Project of the Chinese Academy of Sciences ; Henan Provincial Major Science and Technology Project ; National Natural Science Foundation of China ; Youth Innovation Promotion Association CAS ; Key Research and Development Program of Anhui Province |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/135791]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Hou, Jinyan; Wu, Lifang |
| 作者单位 | 1.Univ Sci & Technol China, Sch Life Sci, Hefei, Anhui, Peoples R China 2.Chinese Acad Sci, Hefei Inst Phys Sci, Ctr Ion Beam Bioengn & Green Agr, Hefei, Peoples R China 3.Chinese Acad Sci, Hefei Inst Phys Sci, Taihe Expt Stn, Fuyang, Anhui, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Dacheng,Su, Pengfei,Gao, Yameng,et al. Efficient plant regeneration through direct shoot organogenesis and two-step rooting in Eucommia ulmoides Oliver[J]. FRONTIERS IN PLANT SCIENCE,2024,15. |
| APA | Wang, Dacheng.,Su, Pengfei.,Gao, Yameng.,Chen, Xue.,Kan, Wenjie.,...&Wu, Lifang.(2024).Efficient plant regeneration through direct shoot organogenesis and two-step rooting in Eucommia ulmoides Oliver.FRONTIERS IN PLANT SCIENCE,15. |
| MLA | Wang, Dacheng,et al."Efficient plant regeneration through direct shoot organogenesis and two-step rooting in Eucommia ulmoides Oliver".FRONTIERS IN PLANT SCIENCE 15(2024). |
入库方式： OAI收割
来源：合肥物质科学研究院
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