Evaluation of targeted sequencing for pathogen identification in bone and joint infections: a cohort study from China
文献类型：期刊论文
| 作者 | Zhang, Qiang2 ; Ding, Yonghua2; Ren, Quanzhong2; Zhang, Feng2; Lyu, Guoqiang1; Lu, Tongxin2; Song, Zhen2; Wang, Qing2; Cheng, Yongxiang2; Wang, Jing2
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| 刊名 | ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
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| 出版日期 | 2024-08-22 |
| 卷号 | 23 |
| 关键词 | Diagnosis Infection Targeted nanopore sequencing Bone and joint Tuberculosis Xpert MTB/RIF |
| DOI | 10.1186/s12941-024-00733-z |
| 通讯作者 | Zhang, Qiang(zq-9999@163.com) |
| 英文摘要 | Purpose Bone and joint tuberculosis (BJTB) is a distinct variant of tuberculosis in which clinical diagnosis often leads to relative misdiagnosis and missed diagnoses. This study aimed to evaluate the diagnostic accuracy of the targeted nanopore sequencing (TNPseq) assay for BJTB patients in China. Method The study enrolled a cohort of 163 patients with suspected BJTB. Diagnostic testing was performed using the TNPseq assay on samples including punctured tissue, pus, and blood. The diagnostic accuracy of the TNPseq assay was then compared with that of the T-SPOT and Xpert MTB/RIF assays. Result TNPseq exhibited superior performance in terms of accuracy, demonstrating a sensitivity of 76.3% (95% CI: 71.0-81.6%) and a specificity of 98.8% (95% CI: 93.5-100%) in clinical diagnosis. When evaluated against a composite reference standard, TNPseq demonstrated a sensitivity of 74.4% (95% CI: 69.3-79.5%) and a specificity of 98.8% (95% CI: 93.7-100%). These results exceed the performance of both the T-SPOT and Xpert MTB/RIF tests. Notably, TNPseq demonstrated high specificity and accuracy in puncture specimens, with a sensitivity of 75.0% (95% CI: 70.2-79.8%) and a specificity of 98.3% (95% CI: 92.7-100%), as well as in pus samples, with a sensitivity of 83.3% (95% CI: 78.6-88.1%) and a specificity of 100% (95% CI: 100-100%). Additionally, TNPseq facilitated the detection of mixed infection scenarios, identifying 20 cases of bacterial-fungal co-infection, 17 cases of bacterial-viral co-infection, and two cases of simultaneous bacterial-fungal-viral co-infection. Conclusion TNPseq demonstrated great potential in the diagnosis of BJTB due to its high sensitivity and specificity. The ability of TNPseq to diagnose pathogens and detect drug resistance genes can also guide subsequent treatment. Expanding the application scenarios and scope of TNPseq will enable it to benefit more clinical treatments. |
| WOS关键词 | MYCOBACTERIUM-TUBERCULOSIS ; RESISTANCE ; DIAGNOSIS ; ASSAYS |
| 资助项目 | Jinan Health Commission Science and Technology Plan ; Public Health Clinical Center affiliated to Shandong University |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:001296587300002 |
| 出版者 | BMC |
| 资助机构 | Jinan Health Commission Science and Technology Plan ; Public Health Clinical Center affiliated to Shandong University |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/135887]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Zhang, Qiang |
| 作者单位 | 1.Second Peoples Hosp Wenshang, Dept Orthoped, Jining 272500, Peoples R China 2.Shandong Univ, Publ Hlth Clin Ctr, Wen Hua Xi Lu 44, Jinan 250100, Shandong, Peoples R China 3.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Hlth & Med Technol, Anhui Prov Key Lab Med Phys & Technol, Hefei 230031, Anhui, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Qiang,Ding, Yonghua,Ren, Quanzhong,et al. Evaluation of targeted sequencing for pathogen identification in bone and joint infections: a cohort study from China[J]. ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS,2024,23. |
| APA | Zhang, Qiang.,Ding, Yonghua.,Ren, Quanzhong.,Zhang, Feng.,Lyu, Guoqiang.,...&Gu, Hongcang.(2024).Evaluation of targeted sequencing for pathogen identification in bone and joint infections: a cohort study from China.ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS,23. |
| MLA | Zhang, Qiang,et al."Evaluation of targeted sequencing for pathogen identification in bone and joint infections: a cohort study from China".ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS 23(2024). |
入库方式： OAI收割
来源：合肥物质科学研究院
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