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Distinguish Esophageal Cancer Cells through VOCs Induced by Methionine Regulation

文献类型：期刊论文

作者Zhou, Jijuan2,3; Ge, Dianlong1,2; Chu, Yajing2,3; Liu, Yue2,3; Lu, Yan1,2; Chu, Yannan1,2
刊名JOURNAL OF PROTEOME RESEARCH
出版日期2024-06-12
关键词esophageal cancer cell methionine regulation volatile organic compound GC-MS untargetedanalysis targeted analysis
ISSN号1535-3893
DOI10.1021/acs.jproteome.4c00270
通讯作者Ge, Dianlong(gedl@cmpt.ac.cn) ; Chu, Yannan(ychu@aiofm.ac.cn)
英文摘要Detection of exhaled volatile organic compounds (VOCs) is promising for noninvasive screening of esophageal cancer (EC). Cellular VOC analysis can be used to investigate potential biomarkers. Considering the crucial role of methionine (Met) during cancer development, exploring associated abnormal metabolic phenotypes becomes imperative. In this work, we employed headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) to investigate the volatile metabolic profiles of EC cells (KYSE150) and normal esophageal epithelial cells (HEECs) under a Met regulation strategy. Using untargeted approaches, we analyzed the metabolic VOCs of the two cell types and explored the differential VOCs between them. Subsequently, we utilized targeted approaches to analyze the differential VOCs in both cell types under gradient Met culture conditions. The results revealed that there were five/six differential VOCs between cells under Met-containing/Met-free culture conditions. And the difference in levels of two characteristic VOCs (1-butanol and ethyl 2-methylbutyrate) between the two cell types intensified with the increase of the Met concentration. Notably, this is the first report on VOC analysis of EC cells and the first to consider the effect of Met on volatile metabolic profiles. The present work indicates that EC cells can be distinguished through VOCs induced by Met regulation, which holds promise for providing novel insights into diagnostic strategies.
WOS关键词EXHALED BREATH ; VOLATILE ; BIOMARKERS
资助项目National Natural Science Foundation of China[21876176] ; National Natural Science Foundation of China[YZJJZX202009] ; President Foundation of Hefei Institute of Physical Sciences of Chinese Academy of Sciences
WOS研究方向Biochemistry & Molecular Biology
语种英语
WOS记录号WOS:001246053600001
出版者AMER CHEMICAL SOC
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; President Foundation of Hefei Institute of Physical Sciences of Chinese Academy of Sciences
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/136160]  
专题中国科学院合肥物质科学研究院
通讯作者Ge, Dianlong; Chu, Yannan
作者单位1.Chinese Acad Sci, Hefei Canc Hosp, Hefei 230031, Anhui, Peoples R China
2.Chinese Acad Sci, Inst Hlth & Med Technol, Hefei Inst Phys Sci, Anhui Prov Key Lab Med Phys & Technol, Hefei 230031, Anhui, Peoples R China
3.Univ Sci & Technol China, Hefei 230026, Anhui, Peoples R China
推荐引用方式
GB/T 7714		 Zhou, Jijuan,Ge, Dianlong,Chu, Yajing,et al. Distinguish Esophageal Cancer Cells through VOCs Induced by Methionine Regulation[J]. JOURNAL OF PROTEOME RESEARCH,2024.
APA Zhou, Jijuan,Ge, Dianlong,Chu, Yajing,Liu, Yue,Lu, Yan,&Chu, Yannan.(2024).Distinguish Esophageal Cancer Cells through VOCs Induced by Methionine Regulation.JOURNAL OF PROTEOME RESEARCH.
MLA Zhou, Jijuan,et al."Distinguish Esophageal Cancer Cells through VOCs Induced by Methionine Regulation".JOURNAL OF PROTEOME RESEARCH (2024).

入库方式： OAI收割

来源：合肥物质科学研究院

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