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Identification of a novel anti-ROR1 nanobody through phage display and its biochemical characterization

文献类型：期刊论文

作者Kang, Li2,3; Dong, Yingkui2,3; Wang, Wanxue3,4; Li, Zehua3; Wang, Yizhuo3,5; Yan, Li3,4; Yin, Cunlong3,4; Zhang, Xiaohui1; Dai, Han3,7; Wu, Bo3,7
刊名BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
出版日期2024-06-22
关键词nanobody phage display ROR1 Wnt5a
ISSN号0885-4513
DOI10.1002/bab.2623
通讯作者Dai, Han(daihan@hmfl.ac.cn) ; Wu, Bo(wubo@hmfl.ac.cn) ; Zhao, Hongxin(zhx@hmfl.ac.cn) ; Wang, Junfeng(junfeng@hmfl.ac.cn)
英文摘要In this study, we aimed to develop nanobodies targeting receptor tyrosine kinase-like orphan receptor 1 (ROR1) for cancer diagnosis and therapy. We immunized alpacas with ROR1, extracted RNA from their blood, and converted it to complementary DNA (cDNA) to amplify the VHH (variable domain of heavy-chain antibodies) sequence. This sequence was used to construct a phage library with a capacity of 8 x108. Screening identified a high-affinity nanobody, HCAbs1, which binds effectively to ROR1. ELISA and surface plasmon resonance analyses revealed HCAbs1's binding affinities to ROR1 at 4.42 and 12.9 nM, respectively. Functional tests showed HCAbs1 could reduce extracellular signal-regulated kinase (ERK) phosphorylation levels induced by Wnt5a in ROR1-transfected cells. Our findings highlight the potential of HCAbs1 nanobodies in diagnosing and treating cancers through targeting ROR1.
WOS关键词ROR1 ; EXPRESSION
资助项目Anhui Major Basic Research Projects ; High Magnetic Field Laboratory of the Chinese Academy of Sciences
WOS研究方向Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
语种英语
WOS记录号WOS:001251499600001
出版者WILEY
资助机构Anhui Major Basic Research Projects ; High Magnetic Field Laboratory of the Chinese Academy of Sciences
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/136508]  
专题中国科学院合肥物质科学研究院
通讯作者Dai, Han; Wu, Bo; Zhao, Hongxin; Wang, Junfeng
作者单位1.Anhui Normal Univ, Coll Life Sci, Wuhu, Anhui, Peoples R China
2.Anhui Univ, Inst Phys Sci & Informat Technol, Hefei, Anhui, Peoples R China
3.Chinese Acad Sci, Hefei Inst Phys Sci, High Magnet Field Lab, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei, Anhui, Peoples R China
4.Anhui Med Univ, Sch Basic Med Sci, Hefei, Anhui, Peoples R China
5.Univ Sci & Technol China, Hefei, Anhui, Peoples R China
6.Hefei China Sci Longwood Biol Technol Co Ltd, Hefei 230088, Anhui, Peoples R China
7.Chinese Acad Sci, Hefei Inst Phys Sci, High Magnet Field Lab, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei, Anhui, Peoples R China
8.Anhui Univ, Inst Phys Sci & Informat Technol, Hefei, Anhui, Peoples R China
推荐引用方式
GB/T 7714		 Kang, Li,Dong, Yingkui,Wang, Wanxue,et al. Identification of a novel anti-ROR1 nanobody through phage display and its biochemical characterization[J]. BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,2024.
APA Kang, Li.,Dong, Yingkui.,Wang, Wanxue.,Li, Zehua.,Wang, Yizhuo.,...&Wang, Junfeng.(2024).Identification of a novel anti-ROR1 nanobody through phage display and its biochemical characterization.BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY.
MLA Kang, Li,et al."Identification of a novel anti-ROR1 nanobody through phage display and its biochemical characterization".BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY (2024).

入库方式： OAI收割

来源：合肥物质科学研究院

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