Sevoflurane enhances autophagy via Rac1 to attenuate lung ischaemia-reperfusion injury
文献类型：期刊论文
| 作者 | Ding, Xian6; Gao, Xiang5 ; Ren, Aolin4; Xu, Jingjing3; Jiang, Xuliang1; Liang, Xiao4; Xie, Kangjie2; Zhou, Yan3; Hu, Chunxiao3; Huang, Dongxiao4
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| 刊名 | CHEMICO-BIOLOGICAL INTERACTIONS
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| 出版日期 | 2024-07-01 |
| 卷号 | 397 |
| 关键词 | Lung ischaemia-reperfusion injury Sevoflurane Rac1 Autophagy |
| ISSN号 | 0009-2797 |
| DOI | 10.1016/j.cbi.2024.111078 |
| 通讯作者 | Huang, Dongxiao(9862023355@jiangnan.edu.cn) |
| 英文摘要 | Sevoflurane can attenuate lung ischaemia-reperfusion injury (LIRI). However, the protective mechanism is unclear. In this study, we developed a LIRI model in vivo that animals (SD, n = 15) were subjected to the administration of 2.2 % sevoflurane 30 min before the onset of left pulmonary artery clamping for 45 min, which was then followed by 60 min of reperfusion treatment. Then, transcriptome sequencing was used to analyse lung tissues. Autophagy inhibition (3 -MA) and Rac1-overexpression transfection plasmids were used in BEAS-2B cells, and BEAS-2B cells were subjected to hypoxia reoxygenation (H/R) and sevoflurane treatment. In both animal tissue and cells, inflammatory cytokines and apoptotic and autophagy molecules were measured by quantitative real-time PCR, western blotting and immunostaining. As a result, decreased arterial partial oxygen and damage to the histological structure of lung tissues were observed in LIRI model rats, and these effects were reversed by sevoflurane treatment. Activation of inflammation (elevated IL-1 beta, IL -6, and TNF-alpha) and apoptosis (elevated cleaved caspase3/caspase3 and Bax, degraded expression of Bcl2) and inhibition of autophagy (elevated P62, degraded expression of Beclin1 and LC3-II/LC3I) in the model group were ameliorated by sevoflurane. Transcriptome sequencing indicated that the PI3K/Akt pathway regulated by Rac1 plays an important role in LIRI. Furthermore, overexpression of Rac1 in a cell line inhibited the protective effect of sevoflurane in LIRI. Autophagy inhibition (3 -MA) also prevented the protective effect of sevoflurane on inflammation and apoptosis. As shown in the present study, sevoflurane enhances autophagy via Rac1/PI3K/AKT signalling to attenuate lung ischaemia-reperfusion injury. |
| WOS关键词 | VOLATILE ANESTHETICS ; INHIBITION ; PROTECTS ; DEXMEDETOMIDINE ; TRANSPLANTATION ; ACTIVATION ; APOPTOSIS ; REDUCE |
| 资助项目 | Top Talent Support Program for Young and Middle-aged People of Wuxi Health Committee[HB2020037] ; Top Talent Support Program for Young and Middle-aged People of Wuxi Health Committee[HB2023025] ; Wuxi Municipal Bureau of Science and Technology[Y20212025] ; Jiangsu Provincial Youth Medical Key Talents Project Fund[QNRC2016201] ; WuXi Taihu Lake Talent Plan, Supports for Leading Talents in Medical and Health Profession ; WuXi key medical discipline construction program from WuXi Health Commission-Innovation Team Of Anaesthesiology[CXTD2021017] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Pharmacology & Pharmacy ; Toxicology |
| 语种 | 英语 |
| WOS记录号 | WOS:001248779600001 |
| 出版者 | ELSEVIER IRELAND LTD |
| 资助机构 | Top Talent Support Program for Young and Middle-aged People of Wuxi Health Committee ; Wuxi Municipal Bureau of Science and Technology ; Jiangsu Provincial Youth Medical Key Talents Project Fund ; WuXi Taihu Lake Talent Plan, Supports for Leading Talents in Medical and Health Profession ; WuXi key medical discipline construction program from WuXi Health Commission-Innovation Team Of Anaesthesiology |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/136520]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Huang, Dongxiao |
| 作者单位 | 1.Fudan Univ, Dept Anesthesiol, Shanghai Canc Ctr, Shanghai 200030, Peoples R China 2.Chinese Acad Sci, Univ Chinese Acad Sci, Zhejiang Canc Hosp,Dept Anesthesiol, Inst Basic Med & Canc,Canc Hosp,Res Ctr Neurooncol, Beijing, Peoples R China 3.Nanjing Med Univ, Affiliated Wuxi Peoples Hosp, Dept Anesthesiol, Nanjing 214023, Peoples R China 4.Jiangnan Univ, Wuxi Peoples Hosp 2, Dept Anesthesiol & Pain Med, Med Ctr, Wuxi 214002, Peoples R China 5.Fujian Med Univ, Affiliated Fujian Matern & Child Hlth Hosp, Dept Anesthesiol, Fuzhou 350001, Peoples R China 6.Jiangnan Univ, Dept Anesthesiol, Affiliated Hosp, Wuxi 214000, Jiangsu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, Xian,Gao, Xiang,Ren, Aolin,et al. Sevoflurane enhances autophagy via Rac1 to attenuate lung ischaemia-reperfusion injury[J]. CHEMICO-BIOLOGICAL INTERACTIONS,2024,397. |
| APA | Ding, Xian.,Gao, Xiang.,Ren, Aolin.,Xu, Jingjing.,Jiang, Xuliang.,...&Huang, Dongxiao.(2024).Sevoflurane enhances autophagy via Rac1 to attenuate lung ischaemia-reperfusion injury.CHEMICO-BIOLOGICAL INTERACTIONS,397. |
| MLA | Ding, Xian,et al."Sevoflurane enhances autophagy via Rac1 to attenuate lung ischaemia-reperfusion injury".CHEMICO-BIOLOGICAL INTERACTIONS 397(2024). |
入库方式： OAI收割
来源：合肥物质科学研究院
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