Multifunctional Laser-Induced Graphene-Based Microfluidic Chip for High-Performance Oocyte Cryopreservation with Low Concentration of Cryoprotectants
文献类型：期刊论文
| 作者 | Li, Yifang1,2,3; Zhang, Jixiang1,2,3; Han, Wei1,2,3 ; Liu, Bianhua1,2; Zhai, Mengjie1,2,3; Li, Nian1,2; Wang, Zhenyang1,2; Zhao, Jun1,2
|
| 刊名 | ADVANCED HEALTHCARE MATERIALS
 |
| 出版日期 | 2024-07-02 |
| 关键词 | DMSO high-performance oocyte cryopreservation ice crystal LIG/PDMS microfluidic chip photothermal effect |
| ISSN号 | 2192-2640 |
| DOI | 10.1002/adhm.202400981 |
| 通讯作者 | Li, Nian(linian@issp.ac.cn) ; Wang, Zhenyang(zywang@iim.ac.cn) ; Zhao, Jun(zjun@iim.ac.cn) |
| 英文摘要 | Oocyte cryopreservation is essential in the field of assisted reproduction, but due to the large size and poor environmental tolerance of oocytes, cell freezing technology needs further improvement. Here, a Y-shaped microfluidic chip based on 3D graphene is ingeniously devised by combining laser-induced graphene (LIG) technology and fiber etching technology. The prepared LIG/PDMS microfluidic chip can effectively suppress ice crystal size and delay ice crystal freezing time by adjusting surface hydrophobicity. In addition, LIG endows the microfluidic chip with an outstanding photothermal effect, which allows to sharply increase its surface temperature from 25 to 71.8 degrees C with 10 s of low-power 808 nm laser irradiation (0.4 W cm-2). Notably, the LIG/PDMS microfluidic chip not only replaces the traditional cryopreservation carriers, but also effectively reduces the dosage of cryoprotectants (CPAs) needed in mouse oocyte cryopreservation. Even when the concentration of CPAs is cut in half (final concentration of 7.5% ethylene glycol (EG) and 7.5% dimethyl sulfoxide (DMSO)), the survival rate of oocytes is still as high as 92.4%, significantly higher than the control group's 85.8%. Therefore, this work provides a novel design strategy to construct multifunctional microfluidic chips for high-performance oocytes cryopreservation. Here, a Y-shaped microfluidic chip based on 3D graphene by combining laser-induced graphene (LIG) technology and fiber etching technology are ingeniously devised. The prepared LIG/PDMS microfluidic chip not only replaces the traditional cryopreservation carriers, but also effectively reduces the dosage of cryoprotectants (CPAs) needed in mouse oocyte cryopreservation and has excellent photothermal bactericidal performance. image |
| WOS关键词 | ICE NUCLEATION ; PRESERVATION ; FABRICATION ; CELLS |
| 资助项目 | National Key Research and Development Project[2020YFA0210703] ; National Key Research and Development Project[2022YFA1203604] ; National Natural Science Foundation of China[52171053] ; National Natural Science Foundation of China[12204488] ; CAS Project for Young Scientists in Basic Research[YSBR-070] ; Key Research and Development Program of Anhui Province[202104a05020036] ; Natural Science Foundation of Anhui Province[2208085ME109] ; HFIPS Director's Fund[YZJJ-GGZX-2022-01] ; HFIPS Director's Fund[GGZX-GTCX-2023-01] ; Joint Training Base Construction Project for Graduate Students in Chongqing[JDLHPYJD2020032] |
| WOS研究方向 | Engineering ; Science & Technology - Other Topics ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:001259680000001 |
| 出版者 | WILEY |
| 资助机构 | National Key Research and Development Project ; National Natural Science Foundation of China ; CAS Project for Young Scientists in Basic Research ; Key Research and Development Program of Anhui Province ; Natural Science Foundation of Anhui Province ; HFIPS Director's Fund ; Joint Training Base Construction Project for Graduate Students in Chongqing |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/136698]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Li, Nian; Wang, Zhenyang; Zhao, Jun |
| 作者单位 | 1.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab Photovolta & Energy Conservat Mat, Hefei 230031, Peoples R China 2.Chinese Acad Sci, Inst Solid State Phys, Hefei Inst Phys Sci, Hefei 230031, Anhui, Peoples R China 3.Chongqing Jiaotong Univ, Sch Mechatron & Vehicle Engn, Chongqing 400074, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Yifang,Zhang, Jixiang,Han, Wei,et al. Multifunctional Laser-Induced Graphene-Based Microfluidic Chip for High-Performance Oocyte Cryopreservation with Low Concentration of Cryoprotectants[J]. ADVANCED HEALTHCARE MATERIALS,2024. |
| APA | Li, Yifang.,Zhang, Jixiang.,Han, Wei.,Liu, Bianhua.,Zhai, Mengjie.,...&Zhao, Jun.(2024).Multifunctional Laser-Induced Graphene-Based Microfluidic Chip for High-Performance Oocyte Cryopreservation with Low Concentration of Cryoprotectants.ADVANCED HEALTHCARE MATERIALS. |
| MLA | Li, Yifang,et al."Multifunctional Laser-Induced Graphene-Based Microfluidic Chip for High-Performance Oocyte Cryopreservation with Low Concentration of Cryoprotectants".ADVANCED HEALTHCARE MATERIALS (2024). |
入库方式： OAI收割
来源：合肥物质科学研究院
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。