A novel competitive color-tone change fluorescence immunochromatographic assay for the ultrasensitive detection of pesticide and veterinary drug residues
文献类型：期刊论文
| 作者 | Wang, Zhenmei3; Zheng, Shuai3; Wang, Chongwen2; Zhang, Long3 ; Liu, Yong1,3; Wu, Xiaosong3; Wang, Shu1,3
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| 刊名 | SENSORS AND ACTUATORS B-CHEMICAL
 |
| 出版日期 | 2024-10-15 |
| 卷号 | 417 |
| 关键词 | Competitive immunoassay Color-tone change Kanamycin Carbendazim |
| DOI | 10.1016/j.snb.2024.136125 |
| 通讯作者 | Liu, Yong(liuyong1s@163.com) ; Wu, Xiaosong(wxs@aiofm.ac.cn) ; Wang, Shu(wangshu87@163.com) |
| 英文摘要 | One important challenge faced by competitive immunoassay techniques is the inability to visually distinguish weak color changes in the T-line during the detection of trace analyte concentrations. In this study, we introduced multi-color fluorescence into the immunoassay system and developed a novel competitive dual-channel color-tone change fluorescent immunochromatographic assay (CFICA) for the visual and quantitative detection of kanamycin (KAN) and carbendazim (CBZ). At the same time, we have developed matching, portable fluorescence-reading equipment, which can analyse the spectral data of the RGB channel individually and can effectively solve the problems of the traditional single-fluorescence reader - large reading error and poor detection sensitivity. After optimization, our CFICA, combined with the corresponding instrument, achieved low limits of detection (LOD) for KAN and CBZ, as low as 1.98 and 12.82 pg/mL, respectively. We believe that this CFICA detection system, with its high accuracy, low error, and multi-color detection capabilities, holds tremendous potential for visual and multi-channel competitive point-of-care testing. |
| 资助项目 | National Natural Science Foundation of China[32200076] ; Natural Science Foundation of Anhui Province[2208085MB29] ; Medical instrument supervise program of Hefei institute of physical science ; CAS[YZJJ2021-J-YQ4] |
| WOS研究方向 | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
| 语种 | 英语 |
| WOS记录号 | WOS:001259580700001 |
| 出版者 | ELSEVIER SCIENCE SA |
| 资助机构 | National Natural Science Foundation of China ; Natural Science Foundation of Anhui Province ; Medical instrument supervise program of Hefei institute of physical science ; CAS |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/136726]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Liu, Yong; Wu, Xiaosong; Wang, Shu |
| 作者单位 | 1.Wan Jiang New Ind Technol Dev Ctr, Tongling 244000, Peoples R China 2.Guangdong Prov Peoples Hosp, Guangdong Acad Med Sci, Dept Clin Lab Med, Guangzhou 510000, Guangdong, Peoples R China 3.Chinese Acad Sci, Hefei Inst Phys Sci, Hefei 230036, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Zhenmei,Zheng, Shuai,Wang, Chongwen,et al. A novel competitive color-tone change fluorescence immunochromatographic assay for the ultrasensitive detection of pesticide and veterinary drug residues[J]. SENSORS AND ACTUATORS B-CHEMICAL,2024,417. |
| APA | Wang, Zhenmei.,Zheng, Shuai.,Wang, Chongwen.,Zhang, Long.,Liu, Yong.,...&Wang, Shu.(2024).A novel competitive color-tone change fluorescence immunochromatographic assay for the ultrasensitive detection of pesticide and veterinary drug residues.SENSORS AND ACTUATORS B-CHEMICAL,417. |
| MLA | Wang, Zhenmei,et al."A novel competitive color-tone change fluorescence immunochromatographic assay for the ultrasensitive detection of pesticide and veterinary drug residues".SENSORS AND ACTUATORS B-CHEMICAL 417(2024). |
入库方式： OAI收割
来源：合肥物质科学研究院
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