白菊木和栌菊木的保护遗传学及亲缘关系研究
文献类型：学位论文
| 作者 | 赵玉娟 |
| 学位类别 | 博士 |
| 答辩日期 | 2011 |
| 授予单位 | 中国科学院研究生院 |
| 导师 | 龚洵 |
| 关键词 | 白菊木 栌菊木 保护遗传学 遗传多样性 居群遗传结构 亲缘关系 |
| 中文摘要 | 白菊木(Leucomeris decora Kurz)和栌菊木(Nouelia insignis Franch.)同属菊科(Asteraceae)帚菊木族(Mutisieae)植物类群，都为我国珍稀濒危植物。本研究基于叶绿体DNA(cpDNA)非编码区序列以及核基因(nDNA)序列的变异，对白菊木和栌菊木的遗传多样性、遗传结构和系统发育进行了研究，并探讨了其相应的保护策略。主要的研究结果如下：1. |
| 学科主题 | Conservation genetics of L. decora: The nucleotide sequences from three chloroplast DNA (cpDNA) regions (the rpl16 intron, trnQ-5’rps16 intergenic spacer and rpl32-trnL intergenic spacer) and two nuclear gene (GAPDH and GBSSI) were selected to characterize the genetic structure and phylogeography of this species. Eleven populations were sampled across the distribution range of L. decora in China. Low levels of cpDNA genetic diversity were found in this species and within populations, with the identification of 2 haplotypes in a total of 2745 bp (cpDNA: π = 0.00102, Hd = 0.468, HT = 0.480), while the levels of genetic diversity revealed by the nuclear gene GAPDH and GBSSI (GAPDH: π = 0.00237, Hd = 0.773, HT = 0.807; GBSSI: π = 0.00167, Hd = 0.751, HT = 0.790) were relatively high, indicating that random losses of genetic polymorphisms from populations may have occurred recently. Relatively high genetic differentiation for both markers (cpDNA: GST = 0.899, FST = 0.893; GAPDH: GST = 0.309, FST = 0.432; GBSSI: GST = 0.274, FST = 0.263) were detected at the wide-species level. Two cpDNA haplotypes, twelve GAPDH haplotypes and ten GBSSI haplotypes were recovered, respectively. Almost every population except the population SP was fixed for one cpDNA haplotype, causing no variation within the populations. Several nuclear haplotypes were distributed widely in most populations. These results may be mainly explained by long-term fragmentation and the limited gene flow caused by geographic isolation among populations. The mismatch distribution analyses and neutrality tests provided no evidence for a recent population expansion in L. decora. The time of all GAPDH haplotypes coalesced to the most recent common ancestor was dated to between 0.46 (95% CI 0.14-0.89)-0.65 (95% CI 0.18-1.22)mya, which predates the LGM. In situ shrinking or extinction might have occurred to L. decora during the glacial ages and L. decora populations did not retreat to common refugia or migrated backwards. Based on the genetic diversity and uniqueness of the populations, conservation strategies are discussed for this endangered species.2. |
| 语种 | 中文 |
| 公开日期 | 2013-10-24 |
| 页码 | 白菊木和栌菊木的遗传结构比较及亲缘关系分析:基于cpDNA的rpl32-trnL以及核基因序列GBSSI对白菊木和栌菊木的遗传多样性以及遗传结构进行比较分析后得出：白菊木的遗传多样性要略高于栌菊木，这可能暗示着白菊木的进化历史要长于栌菊木，或者瓶颈效应在栌菊木中留下了更强的遗传印记所致。两者分布区环境的异质性差异以及花粉流传播的差异导致了白菊木和栌菊木遗传分化水平的差别。两者共有的cpDNA以及核基因单倍型可能是由过去的杂交\基因渐渗或不完全的谱系分选所致。白菊木和栌菊木遗传特征的比较分析表明两者不存在祖先-衍生物种对的关系。 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/16999]  |
| 专题 | 昆明植物研究所_昆明植物所硕博研究生毕业学位论文 |
| 推荐引用方式 GB/T 7714 | 赵玉娟. 白菊木和栌菊木的保护遗传学及亲缘关系研究 [D]. 中国科学院研究生院. 2011. |
入库方式： OAI收割
来源：昆明植物研究所
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