GSDME-mediated pyroptosis promotes anti-tumor immunity of neoadjuvant chemotherapy in breast cancer
文献类型：期刊论文
| 作者 | Fu, Changfang1,5; Ji, Wenbo6; Cui, Qianwen2; Chen, Anling2; Weng, Haiyan7; Lu, Nannan3; Yang, Wulin2,4 |
| 刊名 | CANCER IMMUNOLOGY IMMUNOTHERAPY
 |
| 出版日期 | 2024-07-02 |
| 卷号 | 73 |
| 关键词 | Breast cancer Neoadjuvant chemotherapy Pathological complete response Pyroptosis Immunogenic cell death |
| ISSN号 | 0340-7004 |
| DOI | 10.1007/s00262-024-03752-z |
| 通讯作者 | Lu, Nannan(lnn279@ustc.edu.cn) ; Yang, Wulin(yangw@cmpt.ac.cn) |
| 英文摘要 | Paclitaxel and anthracycline-based chemotherapy is one of the standard treatment options for breast cancer. However, only about 6-30% of breast cancer patients achieved a pathological complete response (pCR), and the mechanism responsible for the difference is still unclear. In this study, random forest algorithm was used to screen feature genes, and artificial neural network (ANN) algorithm was used to construct an ANN model for predicting the efficacy of neoadjuvant chemotherapy for breast cancer. Furthermore, digital pathology, cytology, and molecular biology experiments were used to verify the relationship between the efficacy of neoadjuvant chemotherapy and immune ecology. It was found that paclitaxel and doxorubicin, an anthracycline, could induce typical pyroptosis and bubbling in breast cancer cells, accompanied by gasdermin E (GSDME) cleavage. Paclitaxel with LDH release and Annexin V/PI doubule positive cell populations, and accompanied by the increased release of damage-associated molecular patterns, HMGB1 and ATP. Cell coculture experiments also demonstrated enhanced phagocytosis of macrophages and increased the levels of IFN-gamma and IL-2 secretion after paclitaxel treatment. Mechanistically, GSDME may mediate paclitaxel and doxorubicin-induced pyroptosis in breast cancer cells through the caspase-9/caspase-3 pathway, activate anti-tumor immunity, and promote the efficacy of paclitaxel and anthracycline-based neoadjuvant chemotherapy. This study has practical guiding significance for the precision treatment of breast cancer, and can also provide ideas for understanding molecular mechanisms related to the chemotherapy sensitivity. |
| WOS关键词 | TUMOR-INFILTRATING LYMPHOCYTES ; GENE-EXPRESSION ; PACLITAXEL ; DOXORUBICIN/CYCLOPHOSPHAMIDE ; DOXORUBICIN ; PREDICTOR ; BIOMARKER ; SUBTYPES ; MARKERS |
| 资助项目 | National Natural Science Foundation of China[82203882] ; Hefei Municipal Natural Science Foundation[2022048] ; Hefei Municipal Natural Science Foundation[2022050] ; Anhui University Research Plan[2023AH053392] ; Foundation of Health Commission of Anhui Province[AHWJ2023BAa20159] |
| WOS研究方向 | Oncology ; Immunology |
| 语种 | 英语 |
| WOS记录号 | WOS:001261674200017 |
| 出版者 | SPRINGER |
| 资助机构 | National Natural Science Foundation of China ; Hefei Municipal Natural Science Foundation ; Anhui University Research Plan ; Foundation of Health Commission of Anhui Province |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/136878]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Lu, Nannan; Yang, Wulin |
| 作者单位 | 1.Univ Sci & Technol China, Affiliated Hosp USTC 1, Dept Pharm, Div Life Sci & Med, Hefei 230001, Anhui, Peoples R China 2.Chinese Acad Sci, Inst Hlth & Med Technol, Hefei Inst Phys Sci, Anhui Prov Key Lab Med Phys & Technol, Hefei 230031, Peoples R China 3.Univ Sci & Technol China, Affiliated Hosp USTC 1, Dept Oncol, Div Life Sci & Med, Hefei 230001, Anhui, Peoples R China 4.Chinese Acad Sci, Hefei Canc Hosp, Hefei 230031, Peoples R China 5.Anhui Prov Key Lab Precis Pharmaceut Preparat & Cl, Hefei 230001, Anhui, Peoples R China 6.Anhui Prov Childrens Hosp, Clin Pharm Dept, Hefei 230000, Anhui, Peoples R China 7.Univ Sci & Technol China, Affiliated Hosp USTC 1, Dept Pathol, Div Life Sci & Med, Hefei 230001, Anhui, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fu, Changfang,Ji, Wenbo,Cui, Qianwen,et al. GSDME-mediated pyroptosis promotes anti-tumor immunity of neoadjuvant chemotherapy in breast cancer[J]. CANCER IMMUNOLOGY IMMUNOTHERAPY,2024,73. |
| APA | Fu, Changfang.,Ji, Wenbo.,Cui, Qianwen.,Chen, Anling.,Weng, Haiyan.,...&Yang, Wulin.(2024).GSDME-mediated pyroptosis promotes anti-tumor immunity of neoadjuvant chemotherapy in breast cancer.CANCER IMMUNOLOGY IMMUNOTHERAPY,73. |
| MLA | Fu, Changfang,et al."GSDME-mediated pyroptosis promotes anti-tumor immunity of neoadjuvant chemotherapy in breast cancer".CANCER IMMUNOLOGY IMMUNOTHERAPY 73(2024). |
入库方式： OAI收割
来源：合肥物质科学研究院
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