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SEDphone: Spatial encoding of centrifugal microfluidic disc integrated smartphone-controlled platform via RT/LAMP-CRISPR/Cas12a system for influenza virus subtypes detection

文献类型：期刊论文

作者Yin, Xueer2,3; Wang, Zhenyu2,3; You, Hui4; Shu, Taowei1,2; Hua, Changyi2; Zhao, Jun2; Yang, Ke2; Deng, Guoqing2; Liu, Yong2; Zhu, Ling2
刊名SENSORS AND ACTUATORS B-CHEMICAL
出版日期2024-10-15
卷号417
关键词Centrifugal microfluidic disc Smartphone app-based device CRISPR/Cas12a Loop-mediated isothermal amplification Influenza virus subtypes Multiplex detection
DOI10.1016/j.snb.2024.136196
通讯作者Liu, Yong(liuyong@aiofm.ac.cn) ; Zhu, Ling(zhul@aiofm.ac.cn) ; Zhu, Cancan(zhucancan@aiofm.ac.cn)
英文摘要Accurate and rapid identification of multiple pathogens simultaneously is paramount for global public health, including the detection of different influenza virus subtypes. Recently, the development of CRISPR/Cas technology and its combination with isothermal amplification technology has presented promising opportunities for exploiting point-of-care, highly sensitive and specific detection methods. However, there have been several challenges in terms of multiplex detection and compatibility with isothermal amplification technology, especially loop-mediated isothermal amplification (LAMP). In this study, we introduced a multiplex detection platform, named spatial encoding of centrifugal microfluidic disc integrated smartphone-controlled platform (SEDphone), which was based on CRISPR/Cas12a and RT/LAMP technology. Five distinct influenza virus subtypes could be simultaneously identified: influenza A virus H1N1, H3N2, H5N1, H7N9, and influenza B virus. The assay limit of detection reached 10 copies/mu L within a 45-minute timeframe. Furthermore, the clinical efficacy was validated by detecting 22 positive and 16 negative clinical samples. We anticipate that this simple yet efficient platform holds significant potential as a versatile multiplex pathogen detection in both research and diagnostic settings.
WOS关键词NUCLEIC-ACID DETECTION ; ISOTHERMAL AMPLIFICATION ; CPF1
资助项目National Key Research and Development Program of China[2022YFC2302700] ; Youth Innovation Promotion Association, Chinese Academy of Sciences[Y2022111] ; Dean's Fund of Hefei Institute of Physical Science, China[YZJJ202303- CX]
WOS研究方向Chemistry ; Electrochemistry ; Instruments & Instrumentation
语种英语
WOS记录号WOS:001262095900001
出版者ELSEVIER SCIENCE SA
资助机构National Key Research and Development Program of China ; Youth Innovation Promotion Association, Chinese Academy of Sciences ; Dean's Fund of Hefei Institute of Physical Science, China
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/136893]  
专题中国科学院合肥物质科学研究院
通讯作者Liu, Yong; Zhu, Ling; Zhu, Cancan
作者单位1.Anhui Med Univ, Sch Biomed Engn, Hefei 230032, Peoples R China
2.Chinese Acad Sci, Anhui Inst Opt & Fine Mech, Hefei Inst Phys Sci, Hefei 230031, Peoples R China
3.Univ Sci & Technol China, Hefei 230026, Peoples R China
4.Guangxi Univ, Nanning 530004, Peoples R China
推荐引用方式
GB/T 7714		 Yin, Xueer,Wang, Zhenyu,You, Hui,et al. SEDphone: Spatial encoding of centrifugal microfluidic disc integrated smartphone-controlled platform via RT/LAMP-CRISPR/Cas12a system for influenza virus subtypes detection[J]. SENSORS AND ACTUATORS B-CHEMICAL,2024,417.
APA Yin, Xueer.,Wang, Zhenyu.,You, Hui.,Shu, Taowei.,Hua, Changyi.,...&Zhu, Cancan.(2024).SEDphone: Spatial encoding of centrifugal microfluidic disc integrated smartphone-controlled platform via RT/LAMP-CRISPR/Cas12a system for influenza virus subtypes detection.SENSORS AND ACTUATORS B-CHEMICAL,417.
MLA Yin, Xueer,et al."SEDphone: Spatial encoding of centrifugal microfluidic disc integrated smartphone-controlled platform via RT/LAMP-CRISPR/Cas12a system for influenza virus subtypes detection".SENSORS AND ACTUATORS B-CHEMICAL 417(2024).

入库方式： OAI收割

来源：合肥物质科学研究院

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