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Plasma cell-free DNA methylome-based liquid biopsy for accurate gastric cancer detection

文献类型：期刊论文

作者Qi, Jian4,5,6; Hong, Bo4,5,6; Wang, Shujie4,5,6; Wang, Jingyun3; Fang, Jinman4,5,6; Sun, Ruifang2; Nie, Jinfu1,5,6; Wang, Hongzhi4,5,6
刊名CANCER SCIENCE
出版日期2024-07-22
关键词cell-free DNA cfMeDIP-seq early detection gastric cancer methylation biomarker
ISSN号1347-9032
DOI10.1111/cas.16284
通讯作者Sun, Ruifang(sunruifang@sxmu.edu.cn) ; Nie, Jinfu(jinfunie@163.com) ; Wang, Hongzhi(wanghz@hfcas.ac.cn)
英文摘要Early detection plays a critical role in mitigating mortality rates linked to gastric cancer. However, current clinical screening methods exhibit suboptimal efficacy. Methylation alterations identified from cell-free DNA (cfDNA) present a promising biomarker for early cancer detection. Our study focused on identifying gastric cancer-specific markers from cfDNA methylation to facilitate early detection. We enrolled 150 gastric cancer patients and 100 healthy controls in this study, and undertook genome-wide methylation profiling of cfDNA using cell-free methylated DNA immunoprecipitation and high-throughput sequencing. We identified 21 differentially methylated regions (DMRs) between the gastric tumor and nontumor groups using multiple algorithms. Subsequently, using the 21 DMRs, we developed a gastric cancer detection model by random forest algorithm in the discovery set, and validated the model in an independent set. The model was able to accurately discriminate gastric cancer with a sensitivity and specificity of 93.90% and 95.15% in the discovery set, respectively, and 88.38% and 94.23% in the validation set, respectively. These results underscore the efficacy and accuracy of cfDNA-derived methylation markers in distinguishing early stage gastric cancer. This study highlighted the significance of cfDNA methylation alterations in early gastric cancer detection.
WOS关键词METHYLATION ; POWER
资助项目National Natural Science Foundation of China[81872438] ; Chinese Academy of Sciences
WOS研究方向Oncology
语种英语
WOS记录号WOS:001273835200001
出版者WILEY
资助机构National Natural Science Foundation of China ; Chinese Academy of Sciences
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/137323]  
专题中国科学院合肥物质科学研究院
通讯作者Sun, Ruifang; Nie, Jinfu; Wang, Hongzhi
作者单位1.Chinese Acad Sci, Hong Kong Inst Sci & Innovat, Ctr Regenerat Med & Hlth, Hefei, Peoples R China
2.Shanxi Med Univ, Shanxi Prov Canc Hosp, Chinese Acad Med Sci, Shanxi Hosp,Canc Hosp,Dept Tumor Biobank, 3 Zhigongxincun, Taiyuan 030013, Shanxi, Peoples R China
3.Anhui Univ Sci & Technol, Sch Med, Huainan, Peoples R China
4.Univ Sci & Technol China, Grad Sch, Sci Isl Branch, Hefei, Peoples R China
5.Chinese Acad Sci, Hefei Canc Hosp, Hefei, Peoples R China
6.Chinese Acad Sci, Inst Hlth & Med Technol, Hefei Inst Phys Sci, 350 Shushanhu Rd, Hefei 230031, Anhui, Peoples R China
推荐引用方式
GB/T 7714		 Qi, Jian,Hong, Bo,Wang, Shujie,et al. Plasma cell-free DNA methylome-based liquid biopsy for accurate gastric cancer detection[J]. CANCER SCIENCE,2024.
APA Qi, Jian.,Hong, Bo.,Wang, Shujie.,Wang, Jingyun.,Fang, Jinman.,...&Wang, Hongzhi.(2024).Plasma cell-free DNA methylome-based liquid biopsy for accurate gastric cancer detection.CANCER SCIENCE.
MLA Qi, Jian,et al."Plasma cell-free DNA methylome-based liquid biopsy for accurate gastric cancer detection".CANCER SCIENCE (2024).

入库方式： OAI收割

来源：合肥物质科学研究院

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