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Ultrasensitive Electrochemiluminescence Biosensing Platform Based on Polymer Dots with Aggregation-Induced Emission for Dual-Biotoxin Assay

文献类型：期刊论文

作者Xiang, Shi2; Li, Jingxian2; Wang, Futing2; Yang, Yan2; Yang, Hongfen3; Cai, Ren2; Tan, Weihong1,2,4,5
刊名ACS APPLIED MATERIALS & INTERFACES
出版日期2024-07-11
卷号16
关键词aggregation-induced emission polymer dots ECL aptasensor dual-biotoxin assay sequentialdetection
ISSN号1944-8244
DOI10.1021/acsami.4c08302
通讯作者Yang, Hongfen(yanghf88@gmail.com) ; Cai, Ren(cairen@hnu.edu.cn)
英文摘要Multitarget assay has always been a hot topic in electrochemiluminescence (ECL) methods. Herein, a "on-off-on" ECL aptasensor was developed for the ultrasensitive and sequential detection of possible biological warfare agents, deoxynivalenol (DON) and abrin (ABR). As a luminophore, polymer dots (Pdots) with aggregation-induced emission exhibit high ECL efficiency in the aptasensor, i.e., the signal "on" state. The DON assays mainly depend on ECL quenching due to the efficient quenching effect between ferrocene-H2-ferrocene (Fc-H2-Fc) and Pdots, i.e., the signal "off" state. When the aptasensor is incubated with the oligonucleotide sequence S2 to replace Fc-H2-Fc, obvious ECL recovery occurs, i.e., the signal "on" state, which can be used to sequentially detect ABR. The limit of detection (LOD) for DON is 0.73 fgmL(-1) in the range of 5.0 to 50 ngmL(-1); and the LOD for ABR is similar to 0.38 pgmL(-1) in the range of 1.25 pgmL(-1) to 1.25 mu gmL(-1). The as-designed ECL aptasensor exhibits good stability and reproducibility, high specificity, and favorable practicality. Therefore, this work provides a new approach for assays of DON and ABR in food safety and can be used as a model to design an ultrasensitive ECL biosensor for multitarget detection.
WOS关键词HIGHLY SENSITIVE DETECTION ; ENERGY-TRANSFER ; APTASENSOR ; SYSTEM ; CANCER
资助项目Hunan Provincial Natural Science Foundation of China[2024JJ5348] ; Hunan Provincial Natural Science Foundation of China[2021JJ20020] ; National Natural Science Foundation of China[22004032]
WOS研究方向Science & Technology - Other Topics ; Materials Science
语种英语
WOS记录号WOS:001276280900001
出版者AMER CHEMICAL SOC
资助机构Hunan Provincial Natural Science Foundation of China ; National Natural Science Foundation of China
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/137356]  
专题中国科学院合肥物质科学研究院
通讯作者Yang, Hongfen; Cai, Ren
作者单位1.Shanghai Jiao Tong Univ, Coll Chem & Chem Engn, Shanghai 200240, Peoples R China
2.Hunan Univ, Coll Mat Sci & Engn, Coll Chem & Chem Engn, Mol Sci & Biomed Lab,Coll Biol,State Key Lab Chemo, Changsha 410082, Peoples R China
3.Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 421001, Peoples R China
4.Univ Chinese Acad Sci, Zhejiang Canc Hosp, Hangzhou Inst Med HIM, Chinese Acad Sci,Canc Hosp, Hangzhou 310022, Zhejiang, Peoples R China
5.Shanghai Jiao Tong Univ, Inst Mol Med, Sch Med, Renji Hosp, Shanghai 200240, Peoples R China
推荐引用方式
GB/T 7714		 Xiang, Shi,Li, Jingxian,Wang, Futing,et al. Ultrasensitive Electrochemiluminescence Biosensing Platform Based on Polymer Dots with Aggregation-Induced Emission for Dual-Biotoxin Assay[J]. ACS APPLIED MATERIALS & INTERFACES,2024,16.
APA Xiang, Shi.,Li, Jingxian.,Wang, Futing.,Yang, Yan.,Yang, Hongfen.,...&Tan, Weihong.(2024).Ultrasensitive Electrochemiluminescence Biosensing Platform Based on Polymer Dots with Aggregation-Induced Emission for Dual-Biotoxin Assay.ACS APPLIED MATERIALS & INTERFACES,16.
MLA Xiang, Shi,et al."Ultrasensitive Electrochemiluminescence Biosensing Platform Based on Polymer Dots with Aggregation-Induced Emission for Dual-Biotoxin Assay".ACS APPLIED MATERIALS & INTERFACES 16(2024).

入库方式： OAI收割

来源：合肥物质科学研究院

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