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Surface-Enhanced Raman Scattering-Based Lateral Flow Assay Strips Using Highly Symmetric Gold Nanostars

文献类型：期刊论文

作者Wu, Yixuan2,3; Yang, Qian4; Chen, Jiadong3; Bi, Liyan5; Zhang, Zhiyang1,2; Zhou, Na1,2; Ostovan, Abbas2; Arabi, Maryam2; Chen, Lingxin1,2; Choo, Jaebum3
刊名ACS APPLIED NANO MATERIALS
出版日期2024-12-04
卷号7期号:23页码:27134-27141
关键词lateral flow assay strips symmetric Au nanostar surface-enhanced Raman scattering SERS-LFA strips human IgG detection
DOI10.1021/acsanm.4c05151
通讯作者Chen, Lingxin(lxchen@yic.ac.cn) ; Choo, Jaebum(jbchoo@cau.ac.kr)
英文摘要The applications for lateral flow assay (LFA) strips in point-of-care testing have been significantly constrained by their insufficient sensitivity and reproducibility. To address these inherent issues, we developed surface-enhanced Raman scattering (SERS)-based LFA strips, in which highly symmetric Au nanostars (Sym-AuNS) were employed as the sensing element. Due to the uniform tip-sharp nanostructure and a certain number of branches on the surface of Sym-AuNS, it generates a uniform hotspot distribution, thus producing a strong and stable SERS signal. As a proof of concept, human IgG was chosen as the target to evaluate the performance of the proposed SERS-LFA strips. In human serum spiked samples, the limit of detection for human IgG detection was achieved as low as 38 ng/mL, which exhibited a 2-fold, 3-fold, and 13-fold sensitivity improvement compared with the SERS-LFA strips using conventional gold nanostars (AuNS), enzyme-linked immunosorbent assays (ELISA), and the conventional LFA strips, respectively. Furthermore, the SERS-LFA strips demonstrated high assay reproducibility, with a relative standard deviation of 7.75% for five repeated tests, much lower than those of SERS-LFA strips using AuNS (24.6%), ELISA (12.42%), and conventional LFA strips (31.32%). These results demonstrate that the construction of sensitive and reproducible SERS-LFA strips was obtained, and this platform using Sym-AuNS as SERS nanoparticles paves the way for a promising approach in immunoassay technology.
WOS关键词NANOPARTICLES
WOS研究方向Science & Technology - Other Topics ; Materials Science
语种英语
WOS记录号WOS:001370953800001
资助机构National Natural Science Foundation of China ; National Naturel Science Foundation of China ; National Research Foundation of Korea ; Key Deployment Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Sciences ; Shandong Provincial Natural Science Foundation of China
源URL[http://ir.yic.ac.cn/handle/133337/38133]  
专题烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
烟台海岸带研究所_山东省海岸带环境工程技术研究中心
通讯作者Chen, Lingxin; Choo, Jaebum
作者单位1.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Coastal Zone Ecol Environm Monitoring Technol & Eq, Shandong Key Lab Coastal Environm Proc,Key Lab Coa, Yantai 264003, Peoples R China
3.Chung Ang Univ, Dept Chem, Seoul 06974, South Korea
4.Fujian Agr & Forestry Univ, Coll Food Sci, Fuzhou 350002, Peoples R China
5.Binzhou Med Univ, Sch Special Educ, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714		 Wu, Yixuan,Yang, Qian,Chen, Jiadong,et al. Surface-Enhanced Raman Scattering-Based Lateral Flow Assay Strips Using Highly Symmetric Gold Nanostars[J]. ACS APPLIED NANO MATERIALS,2024,7(23):27134-27141.
APA Wu, Yixuan.,Yang, Qian.,Chen, Jiadong.,Bi, Liyan.,Zhang, Zhiyang.,...&Choo, Jaebum.(2024).Surface-Enhanced Raman Scattering-Based Lateral Flow Assay Strips Using Highly Symmetric Gold Nanostars.ACS APPLIED NANO MATERIALS,7(23),27134-27141.
MLA Wu, Yixuan,et al."Surface-Enhanced Raman Scattering-Based Lateral Flow Assay Strips Using Highly Symmetric Gold Nanostars".ACS APPLIED NANO MATERIALS 7.23(2024):27134-27141.

入库方式： OAI收割

来源：烟台海岸带研究所

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