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Potentiometric enzymatic aptasensing of paralytic shellfish toxins using graphene-wrapped magnetic nanospheres and an all-solid-state cadmium-selective microelectrode

文献类型：期刊论文

作者Li, Jinghui1; Zhang, Wenting1,4; Yin, Tanji1; Qin, Wei1,2,3
刊名SENSORS AND ACTUATORS B-CHEMICAL
出版日期2025-03-01
卷号426页码:7
关键词Paralytic shellfish toxin Potentiometric enzymatic aptasensing Graphene-wrapped magnetic nanospheres Ion-selective microelectrode Deoxyribonuclease I
DOI10.1016/j.snb.2024.137032
通讯作者Qin, Wei(wqin@yic.ac.cn)
英文摘要A highly sensitive potentiometric enzymatic aptasensing system for the detection of paralytic shellfish toxins (PSTs) is developed by integrating graphene-wrapped magnetic nanospheres (Fe3O4@SiO2@rGO) with an allsolid-state ion-selective microelectrode. The multilayered core-shell structure of Fe3O4@SiO2@rGO enhances the efficient adsorption of aptamer molecules tagged with cadmium sulfide (CdS) nanoparticles. These CdSaptamer conjugates, when bound with PST molecules, can be desorbed from the Fe3O4@SiO2@rGO surface into the sample solution and subsequently digested by deoxyribonuclease I (DNase I). This digestion process releases the PST molecules for recirculation, significantly reducing the retention of CdS on the surface of Fe3O4@SiO2@rGO. After magnetic separation and acid dissolution, the released Cd2+ from the magnetic nanospheres are measured using an all-solid-state Cd2+-selective microelectrode, enabling sensitive potentiometric detection of PSTs. Using gonyautoxin 1/4 (GTX 1/4) as a model, the sensing system exhibits a linear concentration range from 5 to 150 pM and a low detection limit of 1.7 pM for GTX 1/4, and has been successfully applied in the analysis of seawater samples.
WOS关键词MICROSPHERES ; COEFFICIENTS ; PROTEINS ; OXIDE
WOS研究方向Chemistry ; Electrochemistry ; Instruments & Instrumentation
语种英语
WOS记录号WOS:001372092600001
资助机构National Natural Science Foundation of China ; Natural Science Foundation of Shandong Province ; Key R & D Program of Shandong Province ; Key Deployment Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Sciences ; Taishan Scholar Program of Shandong Province ; Seed Project of Yantai Institute of Coastal Zone Research
源URL[http://ir.yic.ac.cn/handle/133337/38160]  
专题烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
烟台海岸带研究所_近岸生态与环境实验室
通讯作者Qin, Wei
作者单位1.Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Shandong, Peoples R China
2.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China
4.Yantai Univ, Coll Chem & Chem Engn, Yantai 264005, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Li, Jinghui,Zhang, Wenting,Yin, Tanji,et al. Potentiometric enzymatic aptasensing of paralytic shellfish toxins using graphene-wrapped magnetic nanospheres and an all-solid-state cadmium-selective microelectrode[J]. SENSORS AND ACTUATORS B-CHEMICAL,2025,426:7.
APA Li, Jinghui,Zhang, Wenting,Yin, Tanji,&Qin, Wei.(2025).Potentiometric enzymatic aptasensing of paralytic shellfish toxins using graphene-wrapped magnetic nanospheres and an all-solid-state cadmium-selective microelectrode.SENSORS AND ACTUATORS B-CHEMICAL,426,7.
MLA Li, Jinghui,et al."Potentiometric enzymatic aptasensing of paralytic shellfish toxins using graphene-wrapped magnetic nanospheres and an all-solid-state cadmium-selective microelectrode".SENSORS AND ACTUATORS B-CHEMICAL 426(2025):7.

入库方式： OAI收割

来源：烟台海岸带研究所

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