Insights into the hydrogen-fueled bioreduction of vanadium(V) by marine Shewanella sp. FDA-1: Process and mechanism
文献类型：期刊论文
| 作者 | Cheng, Manman2; Yin, Xin1; Zhang, Haikun2
|
| 刊名 | JOURNAL OF HAZARDOUS MATERIALS
 |
| 出版日期 | 2025-02-05 |
| 卷号 | 483页码:13 |
| 关键词 | Vanadium Bioreduction Marine bacterium Mechanism H-2 |
| ISSN号 | 0304-3894 |
| DOI | 10.1016/j.jhazmat.2024.136585 |
| 通讯作者 | Zhang, Haikun(hkzhang@yic.ac.cn) |
| 英文摘要 | Microbial-driven V(V) reduction plays a crucial role in its biogeochemical cycle, yet the mechanisms underlying this bioreduction remain inadequately understood. While the effectiveness of organic compounds as electron donors in facilitating bacterial reduction of V(V) has been established, the role of inorganic electron donors in initiating this process at the level of pure cultured bacteria has not been explored. In this study, we report on a marine Shewanella sp. FDA-1 that utilizes hydrogen (H-2) as an energy source to reduce V(V). In addition, the reduction mechanism was investigated through a combination of genomics, RT-qPCR, heterologous expression of key proteins, extracellular secretion analyses, and electron transfer activity assays. Our results demonstrate that H-2 serves as an effective electron donor, enabling Shewanella sp. FDA-1 to reduce V(V) across various salinities (2-7 %) and pH values (5-9). When exposed to 5 mM V(V), the presence of 1-20 mL of H-2 resulted in V(V) bioreduction rates ranging from 0.039 to 0.11 h(-1) (R-2 > 0.73). Amorphous V(IV) compounds were characterized as reduction products using XRD, XPS, FTIR, and SEM. Mechanistic studies indicate that the glutathione system, cytochromes, and extracellular substances such as riboflavin play important roles in V(V) reduction (p < 0.05). Furthermore, our findings reveal that the addition of H-2 and lactate triggers different response sequences among these three reduction pathways, suggesting distinct reduction mechanisms between organic and inorganic electron donors. These insights enhance our understanding of microbial vanadium transformation and provide valuable guidance for developing novel H2-based remediation technologies for vanadium-contaminated environments. |
| WOS关键词 | MICROBIAL REDUCTION ; ANTIOXIDANT ; PRECIPITATION ; BACTERIUM ; EBSELEN |
| WOS研究方向 | Engineering ; Environmental Sciences & Ecology |
| 语种 | 英语 |
| WOS记录号 | WOS:001370778600001 |
| 资助机构 | Natural Science Foundation of China ; China Scholarship Council ; Chinese Academy of Sciences |
| 源URL | [http://ir.yic.ac.cn/handle/133337/38148]  |
| 专题 | 海岸带生物学与生物资源利用重点实验室 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 通讯作者 | Zhang, Haikun |
| 作者单位 | 1.Jiangxi Agr Univ, Coll Land Resources & Environm, Nanchang 330045, Jiangxi, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cheng, Manman,Yin, Xin,Zhang, Haikun. Insights into the hydrogen-fueled bioreduction of vanadium(V) by marine Shewanella sp. FDA-1: Process and mechanism[J]. JOURNAL OF HAZARDOUS MATERIALS,2025,483:13. |
| APA | Cheng, Manman,Yin, Xin,&Zhang, Haikun.(2025).Insights into the hydrogen-fueled bioreduction of vanadium(V) by marine Shewanella sp. FDA-1: Process and mechanism.JOURNAL OF HAZARDOUS MATERIALS,483,13. |
| MLA | Cheng, Manman,et al."Insights into the hydrogen-fueled bioreduction of vanadium(V) by marine Shewanella sp. FDA-1: Process and mechanism".JOURNAL OF HAZARDOUS MATERIALS 483(2025):13. |
入库方式： OAI收割
来源：烟台海岸带研究所
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