Improving the Stability and Anti-Infective Activity of Sea Turtle AMPs Using Multiple Structural Modification Strategies
文献类型：期刊论文
| 作者 | Ye, Zifan2,3; Xu, Zhouye3; Ouyang, Jianhong3; Shi, Wenzhuang2,3; Li, Shuangyu2,3; Wang, Xu2,3; Lu, Binjuan3; Wang, Kang3; Wang, Yipeng1,2,3
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| 刊名 | JOURNAL OF MEDICINAL CHEMISTRY
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| 出版日期 | 2024-12-05 |
| 卷号 | 67期号:24页码:22104-22123 |
| ISSN号 | 0022-2623 |
| DOI | 10.1021/acs.jmedchem.4c02039 |
| 通讯作者 | Wang, Yipeng(ypwang@yic.ac.cn) |
| 英文摘要 | Antimicrobial peptides (AMPs) are regarded as promising candidates for combating antimicrobial resistance. Previously we identified an AMP named Cm-CATH2 from the green sea turtle, which exhibited potent antibacterial activity and attractive potential in application. However, natural AMPs including Cm-CATH2 frequently suffer from structural instability and sensitivity to physiological conditions, limiting their effectiveness. Herein, we explored various strategies to enhance the efficacy and stability of Cm-CATH2, including peptide truncation, non-natural amino acid substitutions, disulfide bond-based cyclization, and stapled peptide techniques. The results demonstrated that the truncated NCM4 significantly improved the antimicrobial capability of Cm-CATH2 while also enhancing its anti-inflammatory and antibiofilm activities with minimal cytotoxicity. Further ornithine-substituted peptide oNCM markedly enhanced the stability of NCM4 without compromising its antimicrobial efficacy. This study successfully designed a lead peptide oNCM with significant development potential, while providing valuable insights into the advantages and limitations associated with diverse strategies for enhancing the stability of AMPs. |
| WOS关键词 | PEPTIDE ; RESISTANCE |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:001372602600001 |
| 资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; Key Research & Development Plan in Social Development of Jiangsu Province ; Suzhou Agricultural Science and Technology Innovation Project ; Priority Academic Program Development of the Jiangsu Higher Education Institutes (PAPD) |
| 源URL | [http://ir.yic.ac.cn/handle/133337/38151]  |
| 专题 | 海岸带生物学与生物资源利用重点实验室 海岸带生物资源高效利用研究与发展中心 |
| 通讯作者 | Wang, Yipeng |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China 3.Soochow Univ, Coll Pharmaceut Sci, Dept Biopharmaceut Sci, Suzhou 215123, Jiangsu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ye, Zifan,Xu, Zhouye,Ouyang, Jianhong,et al. Improving the Stability and Anti-Infective Activity of Sea Turtle AMPs Using Multiple Structural Modification Strategies[J]. JOURNAL OF MEDICINAL CHEMISTRY,2024,67(24):22104-22123. |
| APA | Ye, Zifan.,Xu, Zhouye.,Ouyang, Jianhong.,Shi, Wenzhuang.,Li, Shuangyu.,...&Wang, Yipeng.(2024).Improving the Stability and Anti-Infective Activity of Sea Turtle AMPs Using Multiple Structural Modification Strategies.JOURNAL OF MEDICINAL CHEMISTRY,67(24),22104-22123. |
| MLA | Ye, Zifan,et al."Improving the Stability and Anti-Infective Activity of Sea Turtle AMPs Using Multiple Structural Modification Strategies".JOURNAL OF MEDICINAL CHEMISTRY 67.24(2024):22104-22123. |
入库方式： OAI收割
来源：烟台海岸带研究所
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