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Therapeutic effect of phycocyanin on chronic obstructive pulmonary disease in mice

文献类型：期刊论文

作者Li, Wenjun3,4; Li, Yuanyuan1,2; Wang, Qi3,4; Liu, Runze3; Lu, Jianing1; Lu, Wenju1,2; Qin, Song3,4
刊名JOURNAL OF ADVANCED RESEARCH
出版日期2024-12-01
卷号66页码:285-301
关键词Phycocyanin Chronic obstructive pulmonary disease Antioxidants Anti-inflammatory drugs
ISSN号2090-1232
DOI10.1016/j.jare.2024.01.009
通讯作者Lu, Wenju(wlu92@gzhmu.edu.cn) ; Qin, Song(sqin@yic.an.cn)
英文摘要Introduction: The prevention and treatment of chronic obstructive pulmonary disease (COPD) is closely tied to antioxidation and anti-inflammation. Phycocyanin (PC) has numerous pharmacological effects, such as antioxidation and anti-inflammation. However, it remains unclear whether PC can play a therapeutic role in COPD. Objective: As inflammation and oxidative stress can aggravate COPD, this study is to explore the effect of PC on COPD mice and its mechanisms. Methods: The COPD mice model was established by exposing them to lipopolysaccharide (LPS) and cigarette smoke (CS); PC was administrated in a concentration of 50 mg/kg for 30 days. On the last day, lung function was measured, and bronchoalveolar lavage fluid (BALF) was obtained and classified for cells. Lung tissue pathological change was analyzed, and organ indices statistics were measured. Based on molecular docking, the mechanism was explored with Western blotting, immunohistochemical, and immunofluorescence in vivo and in vitro. Results: PC significantly ameliorated the pulmonary function of COPD mice and reduced inflammation of the lung (p < 0.05), and hematoxylin and eosin (H&E) staining showed PC depressed lung inflammatory cell accumulation and emphysema. Periodic acid Schiff (PAS) and Masson staining revealed that PC retarded goblet cells metaplasia and collagen deposition (p < 0.05). In addition, in vivo PC regulated Heme oxygenase 1 (HO-1) (p < 0.05) and NAD(P)H dehydrogenase quinone 1 (NQO1) level (p < 0.01) in the lung, as well as NOX2 level in pulmonary macrophages. Molecular docking results indicate that phycocyanobilin (PCB) in PC had a good binding site in Keap1 and NOX2 proteins; the phycocyanobilin-bound phycocyanin peptide (PCB-PC-peptide) was obtained for further studies. In vitro, PCB-PC-peptide could depress the phospho-NF-E2-related factor 2 (p-Nrf2) and NQO1 protein expression in RAW264.7 cells induced by cigarette smoke extract (CSE) (p < 0.05). Conclusion: PC exerts beneficial effects on COPD via anti-inflammatory and antioxidative stress, which may be achieved through PCB. (c) 2023 The Authors. Published by Elsevier B.V. on behalf of Cairo University. This is an open access articleunder the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
WOS关键词C-PHYCOCYANIN ; RISK-FACTORS ; COPD ; CARBOCISTEINE ; INFLAMMATION ; ANTIOXIDANT ; SMOKING ; INJURY
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:001376641800001
资助机构National Key Research and Development Program of China ; Jilin Province ; Chinese Academy of Sciences for special projects in high technology industrialization
源URL[http://ir.yic.ac.cn/handle/133337/38173]  
专题海岸带生物学与生物资源利用重点实验室
烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
通讯作者Lu, Wenju; Qin, Song
作者单位1.Guangzhou Med Univ, Guangzhou 510030, Peoples R China
2.Guangzhou Med Univ, Natl Clin Res Ctr Resp Dis, State Key Lab Resp Dis, Guangzhou Inst Resp Hlth,Affiliated Hosp 1,State K, Guangzhou 510031, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
4.Shandong Univ Tradit Chinese Med, Jinan 250355, Peoples R China
推荐引用方式
GB/T 7714		 Li, Wenjun,Li, Yuanyuan,Wang, Qi,et al. Therapeutic effect of phycocyanin on chronic obstructive pulmonary disease in mice[J]. JOURNAL OF ADVANCED RESEARCH,2024,66:285-301.
APA Li, Wenjun.,Li, Yuanyuan.,Wang, Qi.,Liu, Runze.,Lu, Jianing.,...&Qin, Song.(2024).Therapeutic effect of phycocyanin on chronic obstructive pulmonary disease in mice.JOURNAL OF ADVANCED RESEARCH,66,285-301.
MLA Li, Wenjun,et al."Therapeutic effect of phycocyanin on chronic obstructive pulmonary disease in mice".JOURNAL OF ADVANCED RESEARCH 66(2024):285-301.

入库方式： OAI收割

来源：烟台海岸带研究所

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