小型集成化自动移液工作站系统及应用
文献类型：期刊论文
| 作者 | 褚亚东 ; 赵宗保
|
| 刊名 | 合成生物学
 |
| 出版日期 | 2022-03 |
| 卷号 | 3期号:1页码:195-208 |
| 英文摘要 | Abstract: Nowadays experiments in biotechnology, synthetic biology and chemical biology often require handling a lot of samples timely with high throughput and accuracy. Manual operations such as liquid dispensing, transferring and distribution not only impose laborious burdens on researchers, but also lead to experimental errors and low efficiency. Therefore, the small-scale and integrated automation systems have emerged, whereby liquid-handling workstation serves as the core to integrate peripheral equipment and coordinate the operation of all instruments through software and human-machine interface. Such systems can be used to handle a large number of labor-consuming workloads and improve efficiency and data quality. As our laboratory is currently running a small-scale liquid handling system, here we first illustrate the major compositions of the system, and then share the processes used for rapid evaluation of culture media and strains as well as enzyme activities for directed evolution, automatic induction, expression and preparation of crude enzyme samples, automatic enzyme-linked immunosorbent assay-based screening and high throughput plasmid extraction. We also briefly introduce key factors that may have significant impact on these processes, such as the location of consumables, the details of pipetting, script extension, and workflow planning. We further describe the shortcomings detected during the use of the system, offer suggestions for assembling similar systems with improved performance, and share the precautions that may cause abnormalities. While integrated automated liquid handling systems are expected to become an important part of the automated laboratory in the future in Chinese universities and research institutes, increased R&D investment and personnel training in hardware and software are needed for promoting the development of domestic laboratory automation equipment. Overall, it is a useful reference for other laboratories to design automated workstation systems |
| 语种 | 中文 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/38250]  |
| 专题 | 海岸带生物学与生物资源利用重点实验室 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 通讯作者 | 赵宗保 |
| 推荐引用方式 GB/T 7714 | 褚亚东,赵宗保. 小型集成化自动移液工作站系统及应用[J]. 合成生物学,2022,3(1):195-208. |
| APA | 褚亚东,&赵宗保.(2022).小型集成化自动移液工作站系统及应用.合成生物学,3(1),195-208. |
| MLA | 褚亚东,et al."小型集成化自动移液工作站系统及应用".合成生物学 3.1(2022):195-208. |
入库方式： OAI收割
来源：烟台海岸带研究所
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