Anthropogenic sources of antimony (Sb) are an important driver of pollution in the Earth environment, but their roles in the historical changes of Sb pollution in lake ecosystems are currently poorly understood. This study documents the sedimentary Sb deposition fluxes in Hongfeng lake (HFL), in southwestern China during 1958–2021 and quantifies the changes of anthropogenic contributions to sediments using Sb stable isotopes. Mean Sb concentration (mean: 1.89 mg kg⁻¹) and deposition flux (mean: 302.1 ng cm⁻² a⁻¹) in lake sediments remained relatively stable from 1958 to 1980. Sb deposition fluxes increased rapidly since 1980, peaked at 990.8 ng cm⁻² a⁻¹ in 2000, and then decreased consistently, reaching 306.9 ng cm⁻² a⁻¹ in 2021. Generally, the historical changes in Sb isotopes were anticorrelated with Sb deposition fluxes and enrichment factors, suggesting a lower ε¹²³Sb signature in anthropogenic loading sources, and highlight the ability of Sb isotopes to distinguish anthropogenic signatures from natural processes in complex hydrological systems. Using a binary end-member mixing model, the contributions of anthropogenic sources to the accumulated of Sb in the lake sediments were estimated to be 20 % before 1980s and increased approximate 58 % during 2000–2015, then decreased to 24 % in 2021, likely reflecting the changes of degree in regional industrial activities. Our results help to better understand the response of Sb pollutions to anthropogenic activities and would in turn benefit the controls Sb contamination in lake ecosystems.