LncRNA pol-lnc78 as a ceRNA regulates antibacterial responses via suppression of pol-miR-n199-3p-mediated SARM down-regulation in Paralichthys olivaceus
文献类型：CNKI期刊论文
| 作者 | Xian-Hui Ning; Bing Han; Ye Peng; Shao-Wu Yin |
| 发表日期 | 2024-01-18 |
| 通讯作者 | Yin, Shao-Wu(yinshaowu@163.com) |
| 出处 | Zoological Research
 |
| 关键词 | LncRNA SARM miRNA ceRNA Antibacterial response |
| 英文摘要 | Long non-coding RNAs(lncRNAs) function as key modulators in mammalian immunity, particularly due to their involvement in lncRNA-mediated competitive endogenous RNA(ceRNA) crosstalk. Despite their recognized significance in mammals, research on lncRNAs in lower vertebrates remains limited. In the present study,we characterized the first immune-related lncRNA(pollnc78) in the teleost Japanese flounder(Paralichthys olivaceus). Results indicated that pol-lnc78 acted as a ceRNA for pol-miR-n199-3p to target the sterile alpha and armadillo motif-containing protein(SARM), the fifth discovered member of the Toll/interleukin 1(IL-1) receptor(TIR) adaptor family. This ceRNA network regulated the antibacterial responses of flounder via the Toll-like receptor(TLR) signaling pathway. Specifically, SARM acted as a negative regulator and exacerbated bacterial infection by inhibiting the expression of inflammatory cytokines IL-1β and tumor necrosis factor-α(TNF-α). PolmiR-n199-3p reduced SARM expression by specifically interacting with the 3' untranslated region(UTR), thereby promoting SARM-dependent inflammatory cytokine expression and protecting the host against bacterial dissemination. Furthermore, pol-lnc78 sponged pol-miRn199-3p to ameliorate the inhibition of SARM expression.During infection, the negative regulators pol-lnc78 and SARM were significantly down-regulated, while pol-miRn199-3p was significantly up-regulated, thus favoring host antibacterial defense. These findings provide novel insights into the mechanisms underlying fish immunity and open new horizons to better understand ceRNA crosstalk in lower vertebrates. |
| DOI标识 | 10.24272/j.issn.2095-8137.2022.520 |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | supported by the National Natural Science Foundation of China (42006082) ; Natural Science Foundation of Jiangsu Province of China (BK20221323) ; “JBGS” Project of Seed Industry Revitalization in Jiangsu Province (JBGS [2021] 034) ; State Key Laboratory of Developmental Biology of Freshwater Fish (2021KF009) |
| 卷 | v.45期:01页:27-37 |
| 语种 | 英文; |
| 出版者 | SCIENCE PRESS |
| 资助项目 | National Natural Science Foundation of China[42006082] ; Natural Science Foundation of Jiangsu Province of China[BK20221323] ; JBGS Project of Seed Industry Revitalization in Jiangsu Province[JBGS [2021] 034] ; State Key Laboratory of Developmental Biology of Freshwater Fish[2021KF009] |
| 分类号 | S917.4 |
| ISSN号 | 2095-8137 |
| WOS记录号 | WOS:001242617600002 |
| WOS研究方向 | Zoology |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/186921]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.CASKeyLaboratoryofExperimentalMarineBiology,CenterforOceanMega-Science,InstituteofOceanology,ChineseAcademyofSciences 2.CollegeofMarineScienceandEngineering,NanjingNormalUniversity 3.Co-InnovationCenterforMarineBio-IndustryTechnologyofJiangsuProvince |
| 推荐引用方式 GB/T 7714 | Xian-Hui Ning,Bing Han,Ye Peng,et al. LncRNA pol-lnc78 as a ceRNA regulates antibacterial responses via suppression of pol-miR-n199-3p-mediated SARM down-regulation in Paralichthys olivaceus. 2024. |
入库方式： OAI收割
来源：海洋研究所
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