Analysis of differentially expressed genes in the sea cucumber Apostichopus japonicus under heat stress
文献类型：CNKI期刊论文
| 作者 | Dongxue Xu ; Jingjing Zhang; Wenqi Song; Lina Sun; Ji Liu; Yuanxue Gu; Yanru Chen; Bin Xia
|
| 发表日期 | 2023-11-15 |
| 出处 | Acta Oceanologica Sinica
 |
| 关键词 | Apostichopus japonicus heat stress RNA-seq protein processing extracellular matrix |
| 英文摘要 | The sea cucumber Apostichopus japonicus plays important roles in marine benthic ecosystem as environmental cleaners, and it is the important aquaculture species in China. High water temperature poses critical threat for the survival of A. japonicus, which has resulted in extensive death in summer. To explore the genes expression profiles under different levels of heat stress, the high-throughput RNA-seq was applied in this study. Our results revealed a total of 1 371, 1 225 and 1 408 differentially expressed genes(DEGs) in 26℃ for 6 h, 26℃ for 48 h and 30℃ for 6 h respectively in comparison with Control group. The pathway analysis suggested “Protein processing in endoplasmic reticulum(ER)” was significantly enriched in all these heat stress(HS) treatment groups. The expression results of key DEGs in this pathway(Hsp70, Derlin, NEF, PDI, GPR94 and ERP57) by qRT-PCR was in accordance with the RNA-seq data. The subcluster analysis of DEGs revealed that a variety of heat shock proteins(HSPs) and calcium ion binding proteins had an obvious up-regulated expression in 26℃ for 6 h, comparatively low expression in 26℃ for 48 h, and the highest expression in 30℃ for 6 h. The other DEGs subcluster, consisting of critical components of extracellular matrix(ECM) and a subset of peptidases and proteases, showed significantly rising tendency in 30℃ for 6 h. Additionally, the expression of matrix metalloproteases(MMP1,MMP16 and MMP19) was prominently affected by HS, and peaked in 30℃ for 6 h. This study provides a series of candidate genes for further study about heat shock response in A. japonicus, especially genes associated with protein processing in ER and regulation of ECM, which also offers new insights into cellular homeostasis under stressful conditions in marine invertebrates. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | The National Natural Science Foundation of China under contract Nos 42276143 and 31902360 ; the Shandong Provincial Natural Science Foundation under contract Nos ZR2022MC050 and ZR2022QD003 ; the “First Class Fishery Discipline” Programme [(2020)3] in Shandong Province |
| 卷 | v.42期:11页:121-130 |
| 语种 | 英文; |
| 分类号 | S917.4 |
| ISSN号 | 0253-505X |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/186955]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.KeyLaboratoryofMaricultureofMinistryofEducation,OceanUniversityofChina 2.SchoolofMarineScienceandEngineering,QingdaoAgriculturalUniversity 3.CASKeyLaboratoryofMarineEcologyandEnvironmentalSciences,InstituteofOceanology,ChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | Dongxue Xu,Jingjing Zhang,Wenqi Song,et al. Analysis of differentially expressed genes in the sea cucumber Apostichopus japonicus under heat stress. 2023. |
入库方式： OAI收割
来源：海洋研究所
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