Oyster studies reveal the duplication and functional diversification of Bivalvia caspase-8 genes
文献类型：CNKI期刊论文
| 作者 | Shaoxi DENG ; Tao QU; Guofan ZHANG; Fei XU
|
| 发表日期 | 2023-05-15 |
| 出处 | Journal of Oceanology and Limnology
 |
| 关键词 | caspase-8 apoptosis metamorphosis inflammation Crassostrea gigas |
| 英文摘要 | Caspase-8, first classified as a pro-apoptotic caspase, is considered to have arisen from duplication with caspase-10 and involves multiple immune and inflammatory responses in mammals.However, few are known on the phylogeny and function of caspase-8 in molluscs, one of the largest phyla in marine invertebrates. In this study, we conducted phylogenetic and functional analysis on molluscan caspase-8-like genes. Results indicate that duplication occurred in molluscan caspase-8-like genes,resulting in at least two caspase-8 copies in some groups of bivalves. Additional studies in Pacific oyster Crassostrea gigas showed different spatio-temporal expression patterns and subcellular localizations of CgCaspase-8-1 and CgCaspase-8-2. While no interaction was observed between CgCaspase-8-2 and CgFADD, the adaptor molecule in apoptosis, yeast two-hybrid and co-immunoprecipitation assays suggested the interaction between CgCaspase-8-1 and CgFADD, indicating its pro-apoptotic function. In addition, CgCaspase-8-1 showed interaction with the CARD domain of CgRIG-I. Together with two NF-κB subunits(Cgp105 and CgRel), their transcripts were up-regulated in response to poly(I:C) stimuli,supporting the immune function of both pro-and anti-inflammation. The results provide insight into the evolution and functional diversification of Bivalvia caspase-8 genes. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the Science&Technology Innovation Project of Laoshan Laboratory (No. LSKJ202203001) ; the Center for Ocean Mega-Research of Science ; Chinese Academy of Sciences (No. COMS2019Q11) ; the GHfund B (No. 20210702) ; the Taishan Scholars Program |
| 卷 | v.41期:03页:281-293 |
| 语种 | 英文; |
| 分类号 | S917.4 |
| ISSN号 | 2096-5508 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/187122]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.NHCKeyLaboratoryofDiagnosisandTherapyofGastrointestinalTumor,GansuProvincialHospital 2.CASandShandongProvinceKeyLaboratoryofExperimentalMarineBiology,CenterforOceanMega-Science,InstituteofOceanology,ChineseAcademyofSciences 3.UniversityofChineseAcademyofSciences 4.LaboratoryforMarineBiologyandBiotechnology,QingdaoNationalLaboratoryforMarineScienceandTechnology |
| 推荐引用方式 GB/T 7714 | Shaoxi DENG,Tao QU,Guofan ZHANG,et al. Oyster studies reveal the duplication and functional diversification of Bivalvia caspase-8 genes. 2023. |
入库方式： OAI收割
来源：海洋研究所
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