Molecular identification,expression pattern,and in-vitro bioactivity analysis of insulin-like growth factor 2 in olive flounder Paralichthys olivaceus
文献类型：CNKI期刊论文
| 作者 | Shuang JIAO; Xungang TAN ; Feng YOU; Shujing ZHANG; Qiuxiang PANG
|
| 发表日期 | 2023-01-15 |
| 出处 | Journal of Oceanology and Limnology
 |
| 关键词 | aquaculture flatfish igf2 proliferation |
| 英文摘要 | Insulin-like growth factors(IGFs) are key regulators of development and growth.Here,we characterized the igf2 gene from olive flounder(Paralichthys olivaceus) and determined its temporal and spatial expression.We set up an in-vitro protein expression system in eukaryotic human embryonic kidney(HEK293 T) cells and explored its effects on cell proliferation.The flounder igf2 cDNA contained a 648-bp open reading frame(ORF) encoding a protein of 215 amino acids(aa),which spanned the complete signal peptide(47 aa),mature peptide(70 aa),and E domain(98 aa).In adult flounder,igf2 mRNA was detected in all selected tissues.In early development,igf2 mRNA was detected throughout development from unfertilized eggs to hatching-stage embryos.In-situ hybridization analysis indicated that igf2 mRNA was specially expressed in the brain region,floor plate,hypochord,otic vesicle,and pectoral fin during embryogenesis.Western blotting analysis indicated that the soluble recombinant flounder IGF2 protein was successfully produced through eukaryotic expression in HEK293T cells.In addition,the recombinant IGF2 protein significantly promoted the proliferation of human cervical carcinoma(HeLa) and HEK293T cells.These results provide new information about the structural and functional conservation,expression patterns,and biological activity of the igf2 in teleosts. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National Natural Science Foundation of China (No. 31972774) ; the National Natural Science Foundation of China (No. 31672636) ; the Key Research and Development Program of Shandong Province,China (No. 2019GHY112007) |
| 卷 | v.41期:01页:282-291 |
| 语种 | 英文; |
| 分类号 | S917.4 |
| ISSN号 | 2096-5508 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/187204]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.Anti-Aging&RegenerativeMedicineResearchInstitution,SchoolofLifeSciencesandMedicine,ShandongUniversityofTechnology 2.CASandShandongProvinceKeyLaboratoryofExperimentalMarineBiology,CenterforOceanMega-Science,InstituteofOceanology,ChineseAcademyofSciences 3.LaboratoryforMarineBiologyandBiotechnology,PilotNationalLaboratoryforMarineScienceandTechnology(Qingdao) |
| 推荐引用方式 GB/T 7714 | Shuang JIAO,Xungang TAN,Feng YOU,et al. Molecular identification,expression pattern,and in-vitro bioactivity analysis of insulin-like growth factor 2 in olive flounder Paralichthys olivaceus. 2023. |
入库方式： OAI收割
来源：海洋研究所
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