Quantitative proteomics provides insight into the response of the marine dinoflagellate Prorocentrum donghaiense to changes in ambient phosphorus
文献类型：CNKI期刊论文
| 作者 | Shufeng ZHANG; Chunjuan YUAN; Ying CHEN; Lin LIN; Dazhi WANG |
| 发表日期 | 2022-03-15 |
| 出处 | Journal of Oceanology and Limnology
 |
| 关键词 | marine dinoflagellates harmful algal blooms Prorocentrum donghaiense phosphorus quantitative proteomics |
| 英文摘要 | Dinoflagellates are the major causative agents of harmful algal blooms in the global ocean and they usually form blooms under conditions of very low dissolved inorganic phosphorus(DIP). However, the mechanisms underpinning the dinoflagellate blooms remain unclear. Here, we quantitatively compared protein expression profiles of a marine dinoflagellate, Prorocentrum donghaiense, grown in inorganic P-replete, P-deficient, and DIP-and dissolved organic phosphorus(DOP)-resupplied conditions by employing a Tandem Mass Tag(TMT)-based quantitative proteomic approach. Proteins involved in intracellular P reallocation, organic P, and non-P lipid utilization were up-regulated under the P-deficient condition, while inorganic phosphate transporters varied insignificantly. In response to the P resupplementation, nitrogen metabolism, ribosome, porphyrin, and chlorophyll metabolism were up-regulated, while lysosome, and starch and sucrose metabolism were down-regulated. Notably, photosynthesis was up-regulated and secondary metabolism was down-regulated only in the DIP-resupplied cells, whereas amino acid metabolism and vitamin B6 metabolism were up-regulated in the DOP-resupplied cells, indicating differential response mechanisms of P. donghaiense to DIP or DOP resupplementation. Our results indicated that P. donghaiense initiated multiple strategies in response to an ambient inorganic P-deficiency, and its effcient DOP assimilation by providing both P and carbon sources might be a key factor driving bloom formations of P. donghaiense in a low DIP environment. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National Key Research Development Program of China (No. 2017YFC1404302) ; the National Natural Science Foundation of China (Nos. 41425021, 41706131) ; the Open Fund of CAS Key Laboratory of Marine Ecology and Environmental Sciences,Institute of Oceanology,Chinese Academy of Sciences (No. KLMEES201806) ; supported by the “Ten-Thousand Talents Program” for leading talents in science and technological innovation |
| 卷 | v.40期:02页:179-192 |
| 语种 | 英文; |
| 分类号 | Q948 |
| ISSN号 | 2096-5508 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/187480]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.KeyLaboratoryofMarineEcology&EnvironmentalSciences,InstituteofOceanology,ChineseAcademyofSciences 2.StateKeyLaboratoryofMarineEnvironmentalScience/CollegeoftheEnvironmentandEcology,XiamenUniversity |
| 推荐引用方式 GB/T 7714 | Shufeng ZHANG,Chunjuan YUAN,Ying CHEN,et al. Quantitative proteomics provides insight into the response of the marine dinoflagellate Prorocentrum donghaiense to changes in ambient phosphorus. 2022. |
入库方式： OAI收割
来源：海洋研究所
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