Screening and characterization of proteases produced by deep-sea cold seep bacteria
文献类型：CNKI期刊论文
| 作者 | Chenchen GUO; Chaomin SUN; Shimei WU |
| 发表日期 | 2022-03-15 |
| 出处 | Journal of Oceanology and Limnology
 |
| 关键词 | deep-sea Bacillus alkaline protease purification characterization |
| 英文摘要 | Fifty protease-producing strains were screened from sediment of deep-sea cold seep, and divided into four different categories: Bacillus, Pseudoalteromonas, Vibrio, and Alteromonas according to the sequences of 16s r RNA. Their abilities to produce protease, amylase, and lipase were determined, and a Bacillus strain gcc-1 displayed very strong alkaline protease activity and stability under different thermal and acidic conditions. The purification of the protease produced by strain gcc-1 was carried out by precipitation with ammonium sulfate, and sequentially chromatographed by anion exchange column and gel filtration. The purified protease showed a single band at the molecular weight of 28 k Da by SDS-PAGE. The characterization results show that the purified protease exhibited a considerable activity and stability in a wide thermal range of 10–80 ℃ and a wide acidic range of p H 6.5–11.5, and displayed highest activity at 40 °C and p H 8.5. Notably, the protease still maintained high activity even at low to 10 ℃. Furthermore, the protease exhibited good stability in presence of different surfactants, organic solvents, oxidizing agent H_2O_2, and commercial detergents. Therefore, the protease produced by gcc-1 is a cold active and high stable enzyme, and has a promising potential in laundry detergent as an additive. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National Natural Science Foundation of China (No. 31600035) to Shimei WU ; the China Ocean Mineral Resources R&D Association Grant (No. DY135-B2-14) to Chaomin SUN |
| 卷 | v.40期:02页:294-305 |
| 语种 | 英文; |
| 分类号 | Q936 |
| ISSN号 | 2096-5508 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/187483]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.CASKeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyofSciences 2.CollegeofLifeSciences,QingdaoUniversity |
| 推荐引用方式 GB/T 7714 | Chenchen GUO,Chaomin SUN,Shimei WU. Screening and characterization of proteases produced by deep-sea cold seep bacteria. 2022. |
入库方式： OAI收割
来源：海洋研究所
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