Genome Editing in the Olive Flounder (Paralichthys olivaceus) Using CRISPR/Cas9 and a Simple Microinjection System
文献类型：CNKI期刊论文
| 作者 | TAN Xungang ; WANG Ling; WU Zhihao; JIAO Shuang; WANG Lijuan; ZOU Yuxia; JIANG Jingteng; YOU Feng
|
| 发表日期 | 2021-12-06 |
| 出处 | Journal of Ocean University of China
 |
| 关键词 | CRISPR/Cas9 gsdf microinjection myomaker needle with brake olive flounder |
| 英文摘要 | The whole-genome sequence of the olive flounder(Paralichthys olivaceus) provides a basis for gene functional analyses,which is important for the aquaculture industry. Understanding gene function will help us to select better economic traits such as fast growth and better culture conditions, which further will increase the aquaculture output. Gene knockout is an important reverse genetics approach for in vivo studies of gene function. In this study, the CRISPR/Cas9 genome editing method with a microinjection system using a simple braked needle was employed in olive flounder. After injection in embryos, green fluorescent protein expression was detected in 40% of larvae. The proportion of normal-hatched larvae was approximately 50%. Different mutations, including short indels and fragment deletions, were found in our test genes gsdf and myomaker. Additionally, we detected more than one mutation in a single larva. In summary, our microinjection technique and CRISPR/Cas9 can be applied to study gene functions in olive flounder. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | supported by the National Natural Science Foundation of China (Nos. 31672636, 31772834, and 31972774) ; the National Key R&D Program of China (Nos. 2018YFD0901202 and 2018YFD0900202) ; the Key Research and Development Program of Shandong Province, China (No. 2019GHY1120070) |
| 卷 | v.20期:06页:237-245 |
| 语种 | 英文; |
| 分类号 | S917.4 |
| ISSN号 | 1672-5182 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/187571]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.ShenghangAquaticScienceandTechnologyCo.,Ltd. 2.CASandShandongProvinceKeyLaboratoryofExperimentalMarineBiology,CenterforOceanMega-Science,InstituteofOceanology,ChineseAcademyofSciences 3.LaboratoryforMarineBiologyandBiotechnology,QingdaoNationalLaboratoryforMarineScienceandTechnology 4.UniversityofChineseAcademyofSciences 5.WeihaiFisheryTechnologyPromotionStation |
| 推荐引用方式 GB/T 7714 | TAN Xungang,WANG Ling,WU Zhihao,et al. Genome Editing in the Olive Flounder (Paralichthys olivaceus) Using CRISPR/Cas9 and a Simple Microinjection System. 2021. |
入库方式： OAI收割
来源：海洋研究所
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