Molecular identification and population differentiation of Aurelia spp. ephyrae in sea cucumber aquaculture ponds of northern China
文献类型：CNKI期刊论文
| 作者 | Saijun PENG; Qingqing LIU; Lei WANG; Tingting SUN; Tamara SHIGANOVA; Zhijun DONG |
| 发表日期 | 2021-05-15 |
| 出处 | Journal of Oceanology and Limnology
 |
| 关键词 | Aurelia coerulea medusae ephyrae 16S r RNA gene analyzes genetic differentiation genetic variability |
| 英文摘要 | Aurelia spp. ephyrae have been reported to form blooms in sea cucumber aquaculture ponds in the Bohai and Yellow Seas. To identify the species, we carried out a genetic analysis of Aurelia spp. ephyrae and medusae based on mitochondrial 16 S rRNA gene. Samples offour Aurelia sp. ephyrae populations were collected in sea cucumber aquaculture ponds and samples offour Aurelia sp. medusae populations were collected in coastal waters. Using a BLASTn search, we found that both the ephyrae collected in the aquaculture ponds and medusae collected in coastal waters belong to Aurelia coerulea. Seventeen haplotypes were recovered from the 16 S rRNA gene. The overall haplotype diversity and nucleotide diversity of the 166 A. coerulea individuals were 0.686% and 0.329%, respectively, indicating high haplotype diversity and low nucleotide diversity. Moreover, the haplotype diversity of ephyrae populations were generally lower than that of medusae populations with close sampling points. The genetic differentiation between ephyrae populations collected in the sea cucumber aquaculture ponds and A. coerulea medusae collected in coastal waters was not significant, suggesting the ephyrae populations in the sea cucumber culture ponds were part of the same genetic group as the medusae populations in the coastal waters. Phylogeographic analysis of the 16 S rRNA region revealed that there was no significant correlation between the haplotypes and the geographic distribution of populations. Pairwise fixation index values showed significant genetic differentiation and limited gene flow between A. coerulea population of Weifang and other locations. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (No. XDA23050301) ; the Special Exchange Program from the Chinese Academy of Sciences ; the Instrument Developing Project of the Chinese Academy of Sciences (No. YJKYYQ20180047) ; the National Natural Science Foundation of China (Nos. 41576152, 41876138) ; the Key Research and Development Program of Yantai (No. 2018ZHGY073) |
| 卷 | v.39期:03页:206-213 |
| 语种 | 英文; |
| 分类号 | S917.4 |
| ISSN号 | 2096-5508 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/187762]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.ShirshovInstituteofOceanologyRussianAcademyofSciences 2.CenterforOceanMega-Science,ChineseAcademyofSciences 3.MupingCoastalEnvironmentResearchStation,YantaiInstituteofCoastalZoneResearch,ChineseAcademyofSciences 4.UniversityofChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | Saijun PENG,Qingqing LIU,Lei WANG,et al. Molecular identification and population differentiation of Aurelia spp. ephyrae in sea cucumber aquaculture ponds of northern China. 2021. |
入库方式： OAI收割
来源：海洋研究所
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