Smyd1 is essential for myosin expression and sarcomere organization in craniofacial, extraocular, and cardiac muscles
文献类型：CNKI期刊论文
| 作者 | Shuang Jiao; Rui Xu ; Shaojun Du
|
| 发表日期 | 2021-03-20 |
| 出处 | Journal of Genetics and Genomics
 |
| 关键词 | Smyd1 Myosin Sarcomere Craniofacial muscle Cardiac muscle |
| 英文摘要 | Skeletal and cardiac muscles are striated myofibers that contain highly organized sarcomeres for muscle contraction. Recent studies revealed that Smyd1, a lysine methyltransferase, plays a key role in sarcomere assembly in heart and trunk skeletal muscles. However, Smyd1 expression and function in craniofacial muscles are not known. Here, we analyze the developmental expression and function of two smyd1 paralogous genes, smyd1 a and smyd1 b, in craniofacial and cardiac muscles of zebrafish embryos. Our data show that loss of smyd1 a(smyd1a~(mb5)) or smyd1 b(smyd1b~(sa15678)) has no visible effects on myogenic commitment and expression of myod and myosin heavy-chain m RNA transcripts in craniofacial muscles.However, myosin heavy-chain protein accumulation and sarcomere organization are dramatically reduced in smyd1b~(sa15678) single mutant, and almost completely diminish in smyd1a~(mb5); smyd1b~(sa15678) double mutant, but not in smyd1a~(mb5) mutant. Similar defects are also observed in cardiac muscles of smyd1b~(sa15678) mutant. Defective craniofacial and cardiac muscle formation is associated with an upregulation of hsp90 a1 and unc45 b m RNA expression in smyd1b~(sa15678) and smyd1a~(mb5); smyd1b~(sa15678) mutants. Together, our studies indicate that Smyd1 b, but not Smyd1 a, plays a key role in myosin heavy-chain protein expression and sarcomere organization in craniofacial and cardiac muscles. Loss of smyd1 b results in muscle-specific stress response. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | supported by funding from the U.S. National Institute of Health (NIH) National Institute of Arthritis and Musculoskeletal and Skin Diseases (R01AR072703 to S. Du) ; supported in part by fellowships from the China Scholarship Council |
| 卷 | v.48期:03页:40-50 |
| 语种 | 英文; |
| 分类号 | Q344 |
| ISSN号 | 1673-8527 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/187808]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.DepartmentofBiochemistryandMolecularBiology,InstituteofMarineandEnvironmentalTechnology,UniversityofMarylandSchoolofMedicine 2.KeyLaboratoryofExperimentalMarineBiology,CenterforOceanMega-Science,InstituteofOceanology,ChineseAcademyofSciences 3.SchoolofLifeSciences,ShandongUniversityofTechnology |
| 推荐引用方式 GB/T 7714 | Shuang Jiao,Rui Xu,Shaojun Du. Smyd1 is essential for myosin expression and sarcomere organization in craniofacial, extraocular, and cardiac muscles. 2021. |
入库方式： OAI收割
来源：海洋研究所
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