Development of high-resolution chloroplast markers for intraspecific phylogeographic studies of Phaeocystis globosa
文献类型：CNKI期刊论文
| 作者 | Qingchun ZHANG; Zhuang NIU; Jinxiu WANG ; Chao LIU; Fanzhou KONG; Xiaokun HU; Jiayu ZHAO; Rencheng YU
|
| 发表日期 | 2021-03-15 |
| 出处 | Journal of Oceanology and Limnology
 |
| 关键词 | Phaeocystis globosa chloroplast DNA marker phylogeny |
| 英文摘要 | Phaeocystis globosa is an important harmful algal bloom causative species distributing widely in temperate and tropical coastal waters in the world.The morphological,physiological,and biochemical characte ristics are different amo ng geographic strains,which can not be distinguished with nuclear ribo somal DNA markers at present.Therefore,the genetic distance and phylogeographic relationships of nuclear 28 S rDNA D1-D2 and ITS regions,and three chloroplast intergenic spacers(petN-trnS1,trnM1-psbA,and rbcS-rpl27) were analyzed and compared among 13 strains of P.globosa isolated from the Pacific Ocean and Atlantic Ocean in this study.In addition,the nucleotide polymorphisms of 28 S rDNA D1-D2,ITS,and rbcS-rpl27 regions were evaluated in two P.globosa strains.The various levels of nucleotide polymorphism were in the nuclear 28 S rDNA D1-D2 region and ITS region,but no polymorphism was in the chloroplast rbcS-rpl27 intergenic spacer.A reasonable intraspecific phylogeographic relationship was presented by rbcS-rpl27 intergenic spacer,which had the strongest distinction to geographic strains compared to those of 28 S rDNA D1-D2 and ITS regions.In the phylogenetic tree of rbcS-rpl27 intergenic spacer,the two strains from the North Sea of the Atlantic Ocean were divided firstly from the species of P.globosa,and then formed an independent clade,while the other Atlantic strains and all of Pacific strains j oined up to build the other clade.It was implied that at least two genetically distant populations of P.globosa existed in the Atlantic coastal regions.This study provided a high-resolution chloroplast marker to analyze intraspecific phylogeographic populations of P.globosa,and preliminarily clarified the genetic relationships of the Pacific and Atlantic strains of P.globosa. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | the National Key Research and Development Plan Project ; the Sino-Australian Centre for Healthy Coasts (No. 2016YFE0101500) ; the Science&Technology Basic Resources Investigation Program (No. 2018FY100206) ; the National Natural Science Foundation of China (Nos. 41576121, 41776127) |
| 卷 | v.39期:02页:125-153 |
| 语种 | 英文; |
| 分类号 | Q948 |
| ISSN号 | 2096-5508 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/187825]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.CASKeyLaboratoryofMarineEcologyandEnvironmentalSciences,InstituteofOceanology,ChineseAcademyofSciences 2.LaboratoryforMarineEcologyandEnvironmentalSciences,PilotNationalLaboratoryforMarineScienceandTechnology(Qingdao) 3.CenterofOceanMega-Science,ChineseAcademyofSciences 4.UniversityofChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | Qingchun ZHANG,Zhuang NIU,Jinxiu WANG,et al. Development of high-resolution chloroplast markers for intraspecific phylogeographic studies of Phaeocystis globosa. 2021. |
入库方式： OAI收割
来源：海洋研究所
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