Detection of two pathogenic marine ciliates Ancistrum haliotis and A. crassum(Ciliophora: Scuticociliatia) by fluorescence in situ hybridization
文献类型：CNKI期刊论文
| 作者 | Zifeng Zhan; Kuidong Xu
|
| 发表日期 | 2020-12-15 |
| 出处 | Acta Oceanologica Sinica
 |
| 关键词 | oligonucleotide probes molecular methods scuticociliates SSU rRNA abalone mollusc |
| 英文摘要 | The scuticociliatid ciliates Ancistrum haliotis and A. crassum are parasites that may cause high mortality in the cultured abalone Haliotis spp. and the bivalve Ruditapes philippinarum. Traditional identification with silver staining methods is hampered by their morphological similarities to closely related species and the complicated procedures of morphological analysis. We designed two SSU rRNA-targeted oligonucleotide probes labeled with a fluorochrome, and optimized the fluorescence in situ hybridization(FISH) protocols for identification of A. halioti and A. crassum, respectively. The assays resulted in a clear identification by strong fluorescence signals from the oligonucleotide probes. The method can be used for quick and accurate quantitative analysis of A. haliotis and A.crassum infections on host molluscs. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | The National Natural Science Foundation of China under contract Nos 41876171 and 41406171 |
| 卷 | v.39期:12页:94-98 |
| 语种 | 英文; |
| 分类号 | S941.5 |
| ISSN号 | 0253-505X |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/187929]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.LaboratoryofMarineOrganismTaxonomyandPhylogeny,InstituteofOceanology,ChineseAcademyofSciences 2.LaboratoryforMarineBiologyandBiotechnology,PilotNationalLaboratoryforMarineScienceandTechnology(Qingdao) 3.CenterforOceanMega-Science,ChineseAcademyofSciences 4.UniversityofChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | Zifeng Zhan,Kuidong Xu. Detection of two pathogenic marine ciliates Ancistrum haliotis and A. crassum(Ciliophora: Scuticociliatia) by fluorescence in situ hybridization. 2020. |
入库方式： OAI收割
来源：海洋研究所
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