Identification and characterization of endosymbiosis-related immune genes in deep-sea mussels Gigantidas platifrons
文献类型：CNKI期刊论文
| 作者 | LI Mengna; CHEN Hao ; WANG Minxiao; ZHONG Zhaoshan; ZHOU Li; LI Chaolun
|
| 发表日期 | 2020-07-15 |
| 出处 | Journal of Oceanology and Limnology
 |
| 关键词 | Gigantidas platifrons endosymbiosis innate immunity pull-down assay immune recognition methane oxidation bacteria |
| 英文摘要 | Deep-sea mussels of the subfamily Bathymodiolinae are common and numerically dominant species widely distributed in cold seeps and hydrothermal vents.During long-time evolution,deep-sea mussels have evolved to be well adapted to the local environment of cold seeps and hydrothermal vents by various ways,especially by establishing endosymbiosis with chemotrophic bacteria.However,biological processes underlying the establishment and maintenance of symbiosis between host mussels and symbionts are largely unclear.In the present study,Gigantidasplatifrons genes possibly involved in the symbio sis with methane oxidation symbionts were identified and characterized by Lipopoly saccharide(LPS) pull-down and in situ hybridization.Five immune related proteins including Toll-like receptor 2(TLR2),integrin,vacuolar sorting protein(VSP),matrix metalloproteinase 1(MMP1),and leucine-rich repeat(LRR-1) were identified by LPS pull-down assay.These five proteins were all conserved in either molecular sequences or functional domains and known to be key molecules in host immune recognition,phagocyto sis,and ly so some-mediated digestion.Furthermore,in situ hybridization of LRR-1,TLR2 and VSP genes was conducted to investigate their expression patterns in gill tissues of G.platifrons.Consequently,LRR-1,TLR2,and VSP genes were found expressed exclusively in the bacteriocytes of G.platifrons.Therefore,it was suggested that TLR2,integrin,VSP,MMP1,and LRR-1 might be crucial molecules in the symbiosis between G.platifrons and methane oxidation bacteria by participating in symbio sis-related immune processes. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (No.XDA22050303) ; the Key Research Program of Frontier Sciences ; CAS (No.ZDBS-LY-DQC032) ; the National Key Research and Development Program of China (No.2018YFC0310800) ; the National Natural Science Foundation of China (No.41906103) ; the Taishan Scholars Project to SUN Song |
| 卷 | v.38期:04页:435-446 |
| 语种 | 英文; |
| 分类号 | S917.4 |
| ISSN号 | 2096-5508 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/188149]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.CenterofDeepSeaResearchandKeyLaboratoryofMarineEcology&EnvironmentalSciences(CODRandKLMEES),InstituteofOceanology,ChineseAcademyofSciences 2.UniversityofChineseAcademyofSciences 3.LaboratoryforMarineEcologyandEnvironmentalScience,QingdaoNationalLaboratoryforMarineScienceandTechnology 4.CenterforOceanMega-Science,ChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | LI Mengna,CHEN Hao,WANG Minxiao,et al. Identification and characterization of endosymbiosis-related immune genes in deep-sea mussels Gigantidas platifrons. 2020. |
入库方式： OAI收割
来源：海洋研究所
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