PI3K/Akt pathway is involved in the activation of RAW 264.7 cells induced by hydroxypropyltrimethyl ammonium chloride chitosan
文献类型：CNKI期刊论文
| 作者 | YANG Yue ; XING Rong'e; LIU Song; QIN Yukun; LI Kecheng; YU Huahua; LI Pengcheng
|
| 发表日期 | 2020-05-15 |
| 出处 | Journal of Oceanology and Limnology
 |
| 关键词 | hydroxypropyltrimethyl ammonium chloride chitosan RAW 264.7 cells PI3K/Akt pathway nuclear factor-κB activating protein 1 |
| 英文摘要 | We previously demonstrated that 2-hydroxypropyltrimethyl ammonium chloride chitosan(HACC) promoted the production of nitric oxide(NO) and proinflammatory cytokines by activating the mitogen-activated protein kinases(MAPK) and Janus kinase(JAK)/STAT pathways in RAW 264.7 cells, indicating good immunomodulatory activity of HACC. In this study, to further investigate the immunomodulatory mechanisms of HACC, we determined the roles of phosphatidylinositol 3-kinase(PI3 K)/Akt, activating protein(AP-1) and nuclear factor kappa B(NF-κB) in HACC-induced activation of RAW 264.7 cells by the western blotting. The results suggest that HACC promoted the phosphorylation of p85 and Akt. Furthermore, c-Jun and p65 were also increased after the treatment of RAW 264.7 cells with HACC, indicating the translocation of NF-κB and AP-1 from cytoplasm to nucleus. In addition, as scanning electron microscopy(SEM) analysis shows, the cell morphology changed after HACC treatment. These findings indicate that HACC activated MAPK, JAK/STAT, and PI3 K/Akt signaling pathways dependent on AP-1 and NF-κB activation in RAW 264.7 cells, ultimately leading to the increase of NO and cytokines. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National Key R&D Program of China (No. 2018YFC0311305) ; the Key Research and Development Program of Shandong Province (Nos. 2019GHY112015,2019YYSP028) |
| 卷 | v.38期:03页:245-251 |
| 语种 | 英文; |
| 分类号 | Q939.91 |
| ISSN号 | 2096-5508 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/188226]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.LaboratoryforMarineDrugsandBioproductsofQingdaoNationalLaboratoryforMarineScienceandTechnology 2.UniversityofChineseAcademyofSciences 3.KeyLaboratoryofExperimentalMarineBiology,CenterforOceanMega-Science,InstituteofOceanology,ChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | YANG Yue,XING Rong'e,LIU Song,et al. PI3K/Akt pathway is involved in the activation of RAW 264.7 cells induced by hydroxypropyltrimethyl ammonium chloride chitosan. 2020. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。