Biosynthesis of phycocyanobilin in recombinant Escherichia coli
文献类型：CNKI期刊论文
| 作者 | MA Chengbo; LI Wenjun; GE Baosheng; LIN Jian; QIN Song |
| 发表日期 | 2020-03-15 |
| 出处 | Journal of Oceanology and Limnology
 |
| 关键词 | combinational biosynthesis phycocyanobilin response surface methodology antioxidant |
| 英文摘要 | The recombinant expression of phycocyanobilin(PCB) was carried out in Escherichia coli,and the best fermentation conditions of recombinant E.coli biosynthesized PCB are optimized in the response surface methodology to improve PCB production.The recombinant PCB is extracted,isolated,and purified by methanol and chloroform extraction.Recombinant PCB is validated in UV-vis spectroscopy,high-pressure liquid chromatography,and mass spectrometry.In addition,the anti-oxidant activities of the recombinant PCB are determined.The best induction conditions that optimized by Design Expert 8.0 software include:lactose concentration 4 mmol/L,induction temperature 24.69℃,induction time 4.6 h,and induction duration 13.57 h,under which the PCB expression level reached approximately 13 mg PCB/L,which is more than four times of previously reported 3 mg PCB/L.The maximum absorption peak of the recombinant PCB is located at 680 nm with a high fluorescence intensity of 470 nm.The recombinant PCB has a good ability to scavenge 2,2-diphenyl-1-picrylhydrazyl(DPPH) free radicals. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National Natural Science Foundation of China(No.41906109) ; the Key Research and Development Program of Yantai(No.Y933021011) |
| 卷 | v.38期:02页:261-270 |
| 语种 | 英文; |
| 分类号 | Q936 |
| ISSN号 | 2096-5508 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/188273]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.YantaiInstituteofCoastalZoneResearch,ChineseAcademyofSciences 2.CollegeofLifeSciences,YantaiUniversity 3.CenterforOceanMega-Science,ChineseAcademyofSciences 4.CenterforBioengineeringandBiotechnology,ChinaUniversityofPetroleum(EastChina) |
| 推荐引用方式 GB/T 7714 | MA Chengbo,LI Wenjun,GE Baosheng,et al. Biosynthesis of phycocyanobilin in recombinant Escherichia coli. 2020. |
入库方式： OAI收割
来源：海洋研究所
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