Establishment and characterization of a new cell line derived from half-smooth tongue sole Cynoglossus semilaevis kidney
文献类型：CNKI期刊论文
| 作者 | LOU Yanan; SUN Bin ; ZHANG Lina; LI Yong; XIAO Peng
|
| 发表日期 | 2020-03-15 |
| 出处 | Journal of Oceanology and Limnology
 |
| 关键词 | kidney cell line Cynoglossus semilaevis viral multiplication |
| 英文摘要 | In this study,we established a new cell line(Cynoglossus semilaevis kidney cells,CSK) from kidney of the half-smooth tongue sole(Cynoglossus semilaevis).The cells were subcultured over 1 000 days and passaged for more than 100 times.Additionally,CSK cells were optimally maintained in Dulbecco's modified Eagle medium nutrient mixture F-12 supplemented with HEPES,antibiotics,fetal bovine serum,2-mercaptoethanol,and basic fibroblast growth factor.The optimum growth temperature for CSK cells was 25℃,and the cells showed a fibroblast-like phenotype.Chromosome analysis revealed that CSK cells had a normal diploid karyotype with 2 n=42.CSK cells were susceptible to Grouper nervous necrosis virus(NNV),and cytopathic ef fects were observed at 3–5 days postinfection.The NNV sensitivity of CSK cells was related to the high abundance of virions in the cytoplasm,as observed by electron microscopy.Additionally,CSK cells could be successfully transfected with a green fluorescent protein reporter plasmid,and fluorescent signals were easily observed.Finally,immunocytochemistry analysis showed that CSK cells were supporting cells.Overall,we established this new cell line,which may have potential applications in the identification of viral pathogens af fecting the half-smooth tongue sole. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National Natural Science Foundation of China(No.31672692) ; the Shinan District Project of Science and Technology Development Fund of Qingdao(No.2016-3-013-ZH) |
| 卷 | v.38期:02页:292-302 |
| 语种 | 英文; |
| 分类号 | S943 |
| ISSN号 | 2096-5508 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/188283]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.LaboratoryforMarineBiologyandBiotechnology,QingdaoNationalLaboratoryforMarineScienceandTechnology 2.TianjinWeishanRoadMiddleSchool 3.KeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,CenterforOceanMega-Science,ChineseAcademyofSciences 4.UniversityofChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | LOU Yanan,SUN Bin,ZHANG Lina,et al. Establishment and characterization of a new cell line derived from half-smooth tongue sole Cynoglossus semilaevis kidney. 2020. |
入库方式： OAI收割
来源：海洋研究所
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