Proof of homothally of Pheopolykrikos hartmannii and details of cyst germination process
文献类型：CNKI期刊论文
| 作者 | CHAI Zhaoyang; HU Zhangxi ; LIU Yuyang; TANG Yingzhong
|
| 发表日期 | 2020-01-15 |
| 出处 | Journal of Oceanology and Limnology
 |
| 关键词 | germination harmful algal blooms (HABs) heterothallism homothallism resting cyst Pheopolykrikos hartmannii |
| 英文摘要 | Resting cysts play crucial roles in the ecology of dino?agellates, especially in wintering or surviving unfavorable conditions, seeding harmful algal blooms(HABs), and facilitating the geographic expansion. Encystment of dino?agellates is tightly coupled with sexual reproduction in most cases, which can occur either through homothallism(self-fertilization) or heterothallism(intercrossing of +/-strains). The types of sexual reproduction have important ecological implications. The toxic and HAB-forming dino?agellate, Pheopolykrikoides hartmannii has been previously reported to be heterothallic. Here, we provide visual con?rmation of homothally of P. hartmannii and the ?rst detailed visual recording of cyst germination based on the observations of a clonal isolate from Jiaozhou Bay, China. To document the homothallism, we ?rst observed cell pairs in sexual mating, planozygotes with two longitudinal ?agella, and cysts with typical morphology as described previously from the clonal culture. We then germinated a single cyst, established a new clonal culture from one of the two daughter cells after the ?rst cell division of the germling(i.e. from the diploid germling to two haploid cells), and produced cysts again from the newly established clonal culture. For the observation of the germination time-series, we took micrographs and videos to show all germination processes, particularly with an interesting observation of the short amoeboid stage of the germling releasing from the archeopyle(～15 s), which was a landmark of the germination process and has not been reported elsewhere. This de?nitive evidence of homothallic sexuality and cyst production in P. hartmannii provides a new insight into the biology and ecology of the species, particularly a mechanism that may partly account for the population dynamics and ubiquitous distribution of the species. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National Key R&D Program of China(No.2017YFC1404300) ; the National Natural Science Foundation of China(Nos.61533011,41776125) ; the NSFC-Shandong Joint Fund for Marine Ecology and Environmental Sciences(No.U1606404) ; the Science and Technology Basic Resources Investigation Program of China(No.2018FY100200) ; the Scientific and Technological Innovation Project of the Qingdao National Laboratory for Marine Science(No.2016ASKJ02) |
| 卷 | v.38期:01页:116-125 |
| 语种 | 英文; |
| 分类号 | Q945 |
| ISSN号 | 2096-5508 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/188340]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.LaboratoryofMarineEcologyandEnvironmentalScience,QingdaoNationalLaboratoryofMarineScienceandTechnology 2.KeyLaboratoryofMarineEcologyandEnvironmentalSciences,InstituteofOceanology,ChineseAcademyofSciences 3.CenterforOceanMega-Science,ChineseAcademyofSciences 4.UniversityofChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | CHAI Zhaoyang,HU Zhangxi,LIU Yuyang,et al. Proof of homothally of Pheopolykrikos hartmannii and details of cyst germination process. 2020. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。