Cloning, characterization and expression analysis of a microsomal glutathione S-transferase gene from the seagrass Zostera marina
文献类型：CNKI期刊论文
| 作者 | Wenjie Yan; Jiao Liu; Samphal Seng; Bin Zhou; Kuke Ding |
| 发表日期 | 2019-10-15 |
| 出处 | Acta Oceanologica Sinica
 |
| 关键词 | Zostera marina antioxidant enzyme glutathione S-transferase(GST) temperature enzyme activity |
| 英文摘要 | The response of glutathione S-transferase(GST) in Zostera marina to temperature variation was analyzed at molecular level by cloning the microsomal GST gene and texting the microsomal GST expression regularity under different temperature. Specific speaking, express Zm GST in Escherichia coli, then purify the recombinant protein and make the thermal stability analysis. Therefore, the experiments were carried out to provide a theoretical basis for the further elaboration to the population degradation mechanisms of Z. marina. In conclusion, the thermostability and the response of Zm GST gene to temperature changes can determine its temperature tolerance range, and affect its resilience in turn. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | The Open Fund of Key Laboratory of Marine Spil Oil Identification and Damage Assessment Technology,State Oceanic Administration under contract No.201704 ; the Shandong Provincial Natural Science Foundation of China under contract No.ZR2018MD020 |
| 卷 | v.38期:10页:115-119 |
| 语种 | 英文; |
| 分类号 | Q943.2 |
| ISSN号 | 0253-505X |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/188433]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.FacultyofFisheries&Aquaculture,RoyalUniversityofAgriculture 2.NationalInstituteforRadiologicalProtection,ChineseCenterforDiseaseControlandPrevention 3.KeyLaboratoryofRadiologicalProtectionandNuclearEmergency,ChineseCenterforDiseaseControlandPrevention 4.InstituteofOceanology,ChineseAcademyofSciences 5.DepartmentofMarineEcology,CollegeofMarineLifeSciences,OceanUniversityofChina |
| 推荐引用方式 GB/T 7714 | Wenjie Yan,Jiao Liu,Samphal Seng,et al. Cloning, characterization and expression analysis of a microsomal glutathione S-transferase gene from the seagrass Zostera marina. 2019. |
入库方式： OAI收割
来源：海洋研究所
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