The transcription of iodothyronine deiodinase genes is regulated by thyroid hormone receptor in the Pacific oyster Crassostrea gigas
文献类型：CNKI期刊论文
| 作者 | HUANG Wen ; XU Fei; LI Li; QUE Huayong; ZHANG Guofan
|
| 发表日期 | 2019-07-15 |
| 出处 | Journal of Oceanology and Limnology
 |
| 关键词 | iodothyronine deiodinase mollusk thyroid hormone(TH) TH responsive elements |
| 英文摘要 | Thyroid hormones(THs)are indispensable for each phyla in Chordata,while their functions in the non-chordate invertebrates are indistinct.Studies on the TH system in non-chordate invertebrates are important for understanding the evolution of TH system and may be applied in aquaculture or biofouling control at the same time.Iodothyronine deiodinases are keys to studying the TH system,as they are critical enzymes in maintaining TH homeostasis by catalyzing the initiation and termination of the effects of thyroid hormone in vertebrates.Here,we report the primary physiological effects of T4,the outer ring deiodinase activity,and a similar transcription regulation of two oyster deiodinases by TH receptor(CgTR)in an invertebrate,Pacific oyster Crassostrea gigas.L-thyroxine(T4)may have an important physiological function in the oyster,suggested by the growth retardation effect of excessive T4 in umbo larvae stage.The outer ring deiodinase activity transforming T4 to T3(3.3',5-triiodothyronine)was then detected in the Pacific oyster in vivo,which may be conducted by two oyster deiodinases(CgDx and CgDy).Transcription regulation of CgTR onto these two deiodinase genes was also verified by electrophoretic mobility shift assay and dual luciferase reporter assay in mammalian cells.These results contribute to a better understanding of the evolution of the TH system. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National Natural Science Foundation of China(No.31372515) ; the Guangxi Natural Science Foundation(Nos.2016GXNSFBA380028,2017GXNSFAA198196) ; the China Agriculture Research System(No.CARS-49) |
| 卷 | v.37期:04页:183-189 |
| 语种 | 英文; |
| 分类号 | S917.4 |
| ISSN号 | 2096-5508 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/188500]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.SchoolofMarineSciences,GuangxiUniversity 2.UniversityofChineseAcademyofSciences 3.CenterforOceanMega-Science,ChineseAcademyofSciences 4.KeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyofSciences 5.LaboratoryforMarineBiologyandBiotechnology,QingdaoNationalLaboratoryforMarineScienceandTechnology |
| 推荐引用方式 GB/T 7714 | HUANG Wen,XU Fei,LI Li,et al. The transcription of iodothyronine deiodinase genes is regulated by thyroid hormone receptor in the Pacific oyster Crassostrea gigas. 2019. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。