Structural analysis and anti-complement activity of polysaccharides extracted from Grateloupia livida (Harv.) Yamada
文献类型：CNKI期刊论文
| 作者 | ZHANG Wenjing; JIN Weihua; DUAN Delin; ZHANG Quanbin |
| 发表日期 | 2019-05-15 |
| 出处 | Journal of Oceanology and Limnology
 |
| 关键词 | red alga Grateloupia livida(Harv.) Yamada mass spectrometry structure |
| 英文摘要 | Polysaccharides were extracted from Grateloupia livida(Harv.) Yamada using hot water(extracted product denoted WGW) and then degraded in dilute sulfuric acid(degraded product denoted WGWD). The degraded mixture was then separated into four fractions through anion exchange chromatography on 2-diethylaminoethanol(DEAE)-Bio-Gel Agarose FF gel. Electrospray ionization collision-induced dissociation tandem mass spectrometry(ESI-CID-MS/MS) was performed to elucidate the structural features of all fractions. In combination with nuclear magnetic resonance spectroscopy(NMR)and infrared spectroscopy(IR) data, the major polysaccharide structures were concluded to be μ-carrageenan and κ-carrageenan. μ-Carrageenan usually has a backbone of alternating 1,3-linked β-D-galactopyranose residues sulfated at C-4 and 1,4-linked a-D-galactopyranose residues sulfated at C-6, while κ-carrageenan consists of alternating 1,3-linked β-D-galactopyranose residues sulfated at C-4 and 1,4-linked a-D-3,6-anhydrogalactopyranose residues. Trace v-carrageenan, composed of 1,3-linked β-D-galactopyranose residues sulfated at C-4 and 1,4-linked a-D-galactopyranose residues sulfated at C-2 and C-6, was also detected. Furthermore, the polysaccharide had a backbone comprising 1,3-linked β-D-galactopyranose and1,4-linked α-L-galactopyranose sulfated at C-6, which is the agarose precursor. The hydroxy groups in the galactopyranose were partially substituted by methyl and pyruvic acid acetal(PA) groups. The anticomplementary activities of WGW and its derivatives against classical pathways were measured. The native polysaccharides in WGW had higher activities, while the derivatives had much weaker activities. This indicated that the molecular weight and sulfate content were important factors affecting the anti-complement activity. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the Ocean Public Welfare Scientific Research Project(No.201405040) ; the National Science and Technology Support Project(No.2013BAB01B01) ; the Special Fund for Cooperation between Guangdong Province and Chinese Academy of Sciences(No.2012B090400028) ; the Science and Technology Program of Applied Basic Research Projects of Qingdao(No.15-9-1-74-jch) |
| 卷 | v.37期:03页:30-38 |
| 语种 | 英文; |
| 分类号 | Q946.3 |
| ISSN号 | 2096-5508 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/188551]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.SchoolofMedicine,ZhejiangUniversity 2.KeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyofSciences 3.CollegeofBiotechnologyandBioengineering,ZhejiangUniversityofTechnology 4.LaboratoryforMarineBiologyandBiotechnology,QingdaoNationalLaboratoryforMarineScienceandTechnology 5.StateKeyLabofSeaweedBioactiveSubstances 6.CenterforOceanMega-Science,ChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | ZHANG Wenjing,JIN Weihua,DUAN Delin,et al. Structural analysis and anti-complement activity of polysaccharides extracted from Grateloupia livida (Harv.) Yamada. 2019. |
入库方式： OAI收割
来源：海洋研究所
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