Identification and characterization of a delta-12 fatty acid desaturase gene from marine microalgae Isochrysis galbana
文献类型：CNKI期刊论文
| 作者 | Xiaotian Han; Shuai Wang; Li Zheng; Wanshun Liu |
| 发表日期 | 2019-02-15 |
| 出处 | Acta Oceanologica Sinica
 |
| 关键词 | delta-12 fatty acid desaturase expression analysis Isochrysis galbana |
| 英文摘要 | The cDNA of the delta-12 fatty acid desaturase gene, IgFAD2, was cloned from the marine microalgae Isochrysis galbana, a species capable of producing docosahexaenoic acid. Sequence analysis indicated that the open reading frame measured a length of 1 158 bp and encoded 386 amino acids with a predicted molecular weight of 42.8 kDa and an isoelectric point of 9.2. Computational analysis of the protein sequence of IgFAD2 showed typical features of membrane-bound desaturase such as three conserved histidine boxes along with four membranespanning regions that were universally present among plant desaturases. Quantitative real-time PCR results showed that the abundance of IgFAD2 transcript was significantly upregulated under different environmental stresses including low temperature(15℃), high salinity(salinity of 62 and 93), and nitrogen starvation(220 μmol/L). Heterologous expression indicated that yeast cells transformed with a plasmid construct containing IgFAD2 could convert endogenous oleic acid(18:1~(?9), OA) into linoleic acid(18:2~(?9, 12), LA). These findings confirm that I. galbana IgFAD2 plays important roles in the biosynthetic pathways of unsaturated fatty acids. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | The Basic Scientific Fund for National Public Research Institutes of China under contract No.2017Q09 ; the Aoshan Science and Technology Innovation Project of Pilot National Laboratory for Marine Science and Technology(Qingdao)under contract No.2016ASKJ02 ; the National Natural Science Foundation of China-Shandong Joint Funded Project under contract No.U1606404 ; the National Natural Science Foundation of China under contract Nos 41776176 and 41806201 ; the 973 Project from Chinese Ministry of Science and Technology under contract No.2015CB755904 ; the Shandong Provincial Natural Science Foundation under contract No.ZR2015PD003 |
| 卷 | v.38期:02页:111-117 |
| 语种 | 英文; |
| 分类号 | Q943.2 |
| ISSN号 | 0253-505X |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/188640]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.CollegeofMarineLifeSciences,OceanUniversityofChina 2.MarineEcologyandEnvironmentalScienceLaboratory,PilotNationalLaboratoryforMarineScienceandTechnology(Qingdao) 3.KeyLaboratoryforMarineBioactiveSubstancesandModernAnalyticalTechnology,FirstInstituteofOceanography,MinistryofNaturalResources 4.ChangjiangEstuaryMarineEcosystemResearchStation,InstituteofOceanology,ChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | Xiaotian Han,Shuai Wang,Li Zheng,et al. Identification and characterization of a delta-12 fatty acid desaturase gene from marine microalgae Isochrysis galbana. 2019. |
入库方式： OAI收割
来源：海洋研究所
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