Neuroanatomy and morphological diversity of brain cells from adult crayfish Cherax quadricarinatus
文献类型：CNKI期刊论文
| 作者 | Duan H(段虎); Jin SJ(金松君); Li FH(李富花); Zhang XJ(张晓军); Xiang JH(相建海)
|
| 发表日期 | 2018-11-15 |
| 出处 | Journal of Oceanology and Limnology
 |
| 关键词 | Cherax quadricarinatus brain's structure brain cells primary culture |
| 英文摘要 | As in vertebrates, brains play key roles in rhythmic regulation, neuronal maintenance, diff erentiation and function, and control of the release of hormones in arthropods. But the structure and functional domains of the brain are still not very clear in crustaceans. In the present study, we reveal the structural details of the brain in the redclaw crayfish using hematoxylin-eosin staining and microscopic examination, firstly. The brain of crayfish is consist of three main parts, namely, protocerebrum, deutocerebrum, and tritocerebrum, including some tracts and commissures, briefly. Secondly, at least 9 kinds of brain cells were identified on the basis of topology and cell shapes, as well as antibody labeling. We also provide morphological details of most cell types, which were previously un-described. In general, four types of glia and three types of neurosecretory cells were described except cluster 9/11 and cluster 10 cells. Glia were categorized into another three main kinds:(1) surface glia;(2) cortex glia; and(3) neuropile glia in addition to astrocytes identified by GFAP labelling. And neurosecretory cells were categorized into I, Ⅱ and III types based on morphological observation. Finally, cluster 9/11 and 10 cells derived from the brain of crayfish, could be used for primary culture about 7–9 d under the optimized conditions. There results provide a resource for improving the knowledge of the still incompletely defined neuroendocrinology of this species. Using the crayfish as an animal model, we are easy to carry out further research in manipulating their endocrine system, exploring cellular and synaptic mechanisms so much as larval production on a small scale, such as in a cell or tissue. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National Natural Science Foundation of China(No.41376165) ; the National Natural Science Foundation of ChinaIsrael Science Foundation(NSFC-ISF)(No.31461143007) ; the Scientific and Technological Innovation Project financially supported by Qingdao National Laboratory for Marine Science and Technology(No.2015ASKJ02) |
| 卷 | v.36期:06页:470-480 |
| 语种 | 英文; |
| 分类号 | S917.4 |
| ISSN号 | 2096-5508 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/188731]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.KeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyofSciences 2.LaboratoryforMarineBiologyandBiotechnology,QingdaoNationalLaboratoryforMarineScienceandTechnology |
| 推荐引用方式 GB/T 7714 | Duan H,Jin SJ,Li FH,et al. Neuroanatomy and morphological diversity of brain cells from adult crayfish Cherax quadricarinatus. 2018. |
入库方式： OAI收割
来源：海洋研究所
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