A potential germ cell-specific marker in Japanese flounder,Paralichthys olivaceus:identification and characterization of lymphocyte antigen 75(Ly75/CD205)
文献类型：CNKI期刊论文
| 作者 | Yang Y(杨阳); Liu QH(刘清华); Ma DY(马道远); Song ZC(宋宗诚); Li J(李军) |
| 发表日期 | 2018-07-15 |
| 出处 | Journal of Oceanology and Limnology
 |
| 关键词 | Ly75/CD205 Japanese flounder Paralichthys olivaceus gonads germ cell-specific marker |
| 英文摘要 | Some germ cell marker genes, such as vasa, nanos, and dead end( dnd), have been identified in fish. Recently, lymphocyte antigen 75(Ly75/CD205) has been identified as a mitotic germ cell-specific cell-surface marker in several fish species. In this study, the Japanese flounder Paralichthys olivaceus ly75 homolog( ly75) was cloned and its expression pattern in gonads was analyzed. The full-length c DNA of ly75 was 7 346 bp, with an open reading frame(ORF) of 5 229 bp. The ORF encoded a protein containing 1 742 amino acids with a predicted molecular mass of 196.89 k Da. In adult tissues, ly75 transcripts were detected in all analyzed tissues but abundantly in the testis. In in-situ hybridization analyses, ly75 mRNA was predominantly localized in oocytes in the ovary and spermatogonia in the testis, but ly75 mRNA was not detected in oogonia, spermatocytes, spermatids, or spermatozoa. These results indicated that ly75 could be a potential germ cell-specific marker in P. olivaceus, as in other fishes. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National Natural Science Foundation of China(Nos.31372514,31472264,31572602) ; the China Agriculture Research System(No.CARS-47) ; the Scientific and Technological Innovation Project Financially Supported by Qingdao National Laboratory for Marine Science and Technology(Nos.2015ASKJ02,2015ASKJ02-03-03) ; the Youth Innovation Promotion Association CAS and Chinese Academy of Science and Technology Service Network Planning(No.KFJ-EW-STS-060) |
| 卷 | v.36期:04页:316-322 |
| 语种 | 英文; |
| 分类号 | S917.4 |
| ISSN号 | 2096-5508 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/188880]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.KeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyofSciences 2.LaboratoryforMarineBiologyandBiotechnology,QingdaoNationalLaboratoryforMarineScienceandTechnology 3.UniversityofChineseAcademyofSciences 4.WeihaiShenghangAquaticProductScienceandTechnologyCo.Ltd. |
| 推荐引用方式 GB/T 7714 | Yang Y,Liu QH,Ma DY,et al. A potential germ cell-specific marker in Japanese flounder,Paralichthys olivaceus:identification and characterization of lymphocyte antigen 75(Ly75/CD205). 2018. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。