Preparation of low molecular weight Sargassum fusiforme polysaccharide and its anticoagulant activity
文献类型：CNKI期刊论文
| 作者 | Sun YH(孙雨豪) ; Chen XL(陈晓琳); Liu S(刘松); Yu HH(于华华); Li RF(李荣锋); Wang XQ(王雪芹); Qin YK(秦玉坤); Li PC(李鹏程)
|
| 发表日期 | 2018-05-15 |
| 出处 | Journal of Oceanology and Limnology
 |
| 关键词 | anticoagulant activity Sargassum fusiforme low molecular weight degradation |
| 英文摘要 | Heparin has been used as an anticoagulant drug for many years, but it has significant side ef fects. In the search for good substitutes, low molecular weight(MW) polysaccharides from S argassum fusiforme have been examined and confi rmed to possess biological activities. Here, S. fusiforme polysaccharides(SFP) were extracted and subjected to a hydrogen peroxide(H_2O_2) oxidation method for the preparation of low-MW SFP(LSFP). The effects of temperature, pH, and H_2O_2 concentration on the degradation process were also examined. Several LSFP of 36, 9, 5.7, and 2.7 kDa were obtained under different conditions, and their anticoagulant activities studied in vitro. The results showed that SFP and LSFP prolonged activated partial thromboplastin(APTT), prothrombin(PT) and thrombin times(TT) significantly, indicating that these low MW polysaccharides possessed anticoagulant activity in the intrinsic, extrinsic, and common coagulation pathways. As these ef fects were related to the MW of the polysaccharides in APTT and TT but not in PT, the contents of the monosaccharide fucose and sulfate and the polysaccharide MW could have exerted combined ef fects. The details of this mechanism require further verifi cation. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the Ocean Public Welfare Scientifi c Research Project,State Oceanic Administration of the People’s Republic of China(No.201505033) ; the Qingdao People’s Livelihood Science and Technology Program(No.16-6-2-41-nsh) |
| 卷 | v.36期:03页:284-293 |
| 语种 | 英文; |
| 分类号 | TQ464.1 |
| ISSN号 | 2096-5508 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/188936]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.InstituteofOceanology,ChineseAcademyofSciences 2.UniversityofChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | Sun YH,Chen XL,Liu S,et al. Preparation of low molecular weight Sargassum fusiforme polysaccharide and its anticoagulant activity. 2018. |
入库方式： OAI收割
来源：海洋研究所
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