Peritrophin-like protein from Litopenaeus vannamei(LvPT) involved in white spot syndrome virus(WSSV) infection in digestive tract challenged with reverse gavage
文献类型：CNKI期刊论文
| 作者 | Xie SY(谢世筠) ; Li FH(李富花); Zhang XJ(张晓军); Zhang JQ(张继泉); Xiang JH(相建海)
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| 发表日期 | 2017-11-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
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| 关键词 | Litopenaeus vannamei digestive tract peritrophin-like protein dsRNA interference binding specificity white spot syndrome virus(WSSV) reverse gavage |
| 英文摘要 | The peritrophic membrane plays an important role in the defense system of the arthropod gut. The digestive tract is considered one of the major tissues targeted by white spot syndrome virus(WSSV) in shrimp. In this study, the nucleotide sequence encoding peritrophin-like protein of L itopenaeus vannamei(Lv PT) was amplified from a yeast two-hybrid library of L. vannamei. The epitope peptide of Lv PT was predicted with the Gen Script Optimum Antigen? design tool. An anti-Lv PT polyclonal antibody was produced and shown to specifically bind a band at ~27 k Da, identified as Lv PT. The Lv PT protein was expressed and its concentration determined. L v PT ds RNA(4 μg per shrimp) was used to inhibit Lv PT expression in shrimp, and a WSSV challenge experiment was then performed with reverse gavage. The pleopods, stomachs, and guts were collected from the shrimp at 0, 24, 48, and 72 h post-infection(hpi). Viral load quantification showed that the levels of WSSV were significantly lower in the pleopods, stomachs, and guts of shrimp after L v PT ds RNA interference than in those of the controls at 48 and 72 hpi. Our results imply that Lv PT plays an important role during WSSV infection of the digestive tract. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National Basic Research Program of China(973 Program)(No.2012CB114403) ; the China Agriculture Research System-47(CARS-47) |
| 卷 | v.35期:06页:266-272 |
| 语种 | 英文; |
| 分类号 | S945.49 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/189125]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.KeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyofSciences 2.UniversityofChineseAcademyofSciences 3.LaboratoryforMarineBiologyandBiotechnology,QingdaoNationalLaboratoryforMarineScienceandTechnology |
| 推荐引用方式 GB/T 7714 | Xie SY,Li FH,Zhang XJ,et al. Peritrophin-like protein from Litopenaeus vannamei(LvPT) involved in white spot syndrome virus(WSSV) infection in digestive tract challenged with reverse gavage. 2017. |
入库方式： OAI收割
来源：海洋研究所
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